Arrayit Microarray Slide Substrates are atomically flat glass, affording a universal high-capacity binding surface for DNA and protein microarray applications and many other life sciences applications that require high quality surface chemistry. The open platform 25 x 76 mm glass substrate slide format renders reactive “amine”, “aldehyde”,  “epoxy” and “NHS” substrate slides compatible with all standard microarrayers and scanners.  If you do not know what surface chemistry will work best for your application, we can design your assay for you.

Glass Technical Specifications:
Arrayit Microarray Substrates are not treated microscope slides!  Atomically flat polished glass is used as the starting point for this product. Glass substrates of this quality routinely retail at $50-100 each from leading optical suppliers.  Users will benefit from the following technical features:

• Glass polished to atomic smoothness (±20 angstroms)
• Only polished glass surface in the microarray industry
• Polishing improves topology and uniformity – keys to precise data
• Homogenous streptavidin proteins across the entire surface provide superior biotin binding
• Manufactured in a state-of-the-art class 1 cleanrooms
• Ultra-low intrinsic fluorescence and background noise
• Open platform dimensions compatible with all major brands of microarrayers and scanners
• Precise physical dimensions (25 ± 0.2 mm x 76 ± 0.3 mm x 0.940 mm ± 0.025 mm)
• Proprietary corner chamfer (1.4 mm) provides unambiguous side and end orientation to simplify printing and processing
• Finished edges enhance user safety
• Excellent refractive index, transmission and hardness specifications
• Sophisticated anti-static packaging improves usability
• Offered with or without bar-codes
• Custom laser and chrome fiducials available upon request
• High-volume 100,000 piece per month manufacturing capabilities
• Product arrives “ready to use” with no additional processing required

Figure 1. PDMS devices can be fabricated to any design.  Email us your specification for a quote, no job is too large or too small, prototyping and fast turn arounds are possible.

Figure 2. Atomic Force Microscopy (AFM) analysis. Shown are AFM scans of the SuperClean Microarray Substrate Slide by Arrayit, Sunnyvale CA. Data are coded to a rainbow intensity scale, such that red data represents 5.0 nm or 50 angstroms. The substrates have an average smoothness of 2.0 nm or 20 angstroms, which corresponds to about 10 silicon dioxide bonds.

Figure 3. Correct Substrate Slide Orientation. Shown is a graphic of two ArrayIt® Microarray Substrates, showing the correct and incorrect orientation for use. In the correct orientation (blue graphic), the chamfer will be located in the upper right corner and samples and PDMS is manufactured on the side facing upward, which is the same side that contains the word “Correct!”. In the incorrect orientation (red graphic), the chamfer will be located in the upper left corner, placing the backside facing upward, which is the side that contains the word  “Incorrect!”. Only one side of ArrayIt® Microarray Substrates is suitable for printing microarrays. Please print on the correct side only.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com(under the subject heading please type "ArrayIt® technical assistance"). By telephone: (408) 744-1331, Monday–Friday PST 9:00am - 6:30pm. Please remember that we want to hear about your successes!

Scientific Publications
Click here and here for recent scientific publications using ArrayIt® brand microarray products from Arrayit International, Inc for streptavidin experimentation.

Recommended Equipment and Reagents
NanoPrint™ 2 Microarrayers
SpotBot® 4 Personal Microarrayers
InnoScan® Microarray Scanners
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuge
Protein Printing Buffer
BlockIt™ Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers