Arrayit AHC4X24 Hybridization Cassettes enable up to 96 hybridization or protein microarray reactions in a 3 x 8 x 4 format. AHC4X24 holds 4 standard glass substrate slides (1 x 25 x 76 mm) and uses a silicone gasket to create 96 individual reaction areas for up to 96 simultaneous microarray reactions. This hybridization cassette utilizes a compression-fit silicone gasket to demarcate the microarray reaction areas, eliminating the need for contaminating adhesives and inks. The 7.5 mm x 6.5 mm reaction areas are configured at 9 mm center-to-center spacing in the 3 x 8 x 4 format for compatibility with all multi-channel pipettes and automated liquid handling robots and workstations. AHC4X24 is precision-machined from durable, flat high-grade aluminum to ensure proper alignment and leak-proof fit. The top portion of the cassette is engraved with a 1-12 number panel across the top and an A-H letter panel down the left side for unambiguous identification of each of the 96 reaction wells. Each AHC4X24 cassette includes an anodized aluminum top and bottom, square cassette wells, SBS compliant top and bottom, a 3 x 8 x 4 well silicone gasket and no septa mat for sealing the wells during use. Wells can be sealed with adhesive foil seals (Cat. MPAFS) if necessary.

Tools - Microarray Hybridization - Multi-Well Microarray Hybridization Cassettes

Arrayit offers innovative hardware solutions for creating multi-well microarrays on a single glass substrate slide. Reaction cassettes containing 8, 16, 24, 48 or 96 wells utilize silicone gaskets to create individual reaction wells encompassing the printed microarrays. The upper portion of the Hybridization Cassette allows the introduction of individual samples, making these cassettes ideal for DNA and protein microarray research and diagnostics.

Figure 1A.The AHC1X24 has 7.5 x 6.5 mm wells spaced at 9 mm centers in a 3 x 8 format for easy loading with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the gasket against the hardware and the thin portion against the glass substrate slide.	Figure 1B.The AHC1X16 has 7.5 x 7.5 mm wells spaced at 9 mm centers in a 2 x 8 format for easy loading with multi-channel pipettes and automation systems. Note that the proper orientation fits the wide portion of the gasket against the hardware and the thin portion against the glass substrate slide.

Figure 2. Diagramatic depiction of the wells formed by Hybridization Cassette 1x24 (left) and 1x16 (right). Use these spacing coordinates to program the microarrayer prior to printing the 3x8 (1x24) or 2x8 (1x16) microarray patterns required to utilize the multi-well Hybridization Cassettes. Use the SpotBot and NanoPrint Microarrayer to simplify the microarray manufacturing using these formats. NOTE! Contact Arrayit for other gaskets with larger reaction areas!

Figure 3. Hybridization Cassette 4x16 (Catalog ID AHC4X16) is identical to Hybridization Cassette 1x16 except that it contains four identical units allowing the use of four glass substrate slide microarrays. The Hybridization Cassette 4x16 has a 7.5 x 7.5 mm well size set at 9 mm centers in four identical 2x8 units. Well openings in the top portion of the Hybridization Cassette (shown here) are 5.5 x 5.5 mm. Requires the use of four individual silicon gaskets (Catalog ID GAHC1x16) to demarcate the individual wells, and the four gaskets are included with the purchase of the AHC4x16 product. Easily sealed with foil seals to prevent evaporation.

Figure 4. Hybridization Cassette 4x24 (Cat. AHC4X24) is identical to AHC1x24 except that it contains four identical units, allowing the use of four glass substrate slides. AHC4x24 has a 7.5 x 6.5 mm well size set at 9 mm centers in four identical 3x8 units. Well openings in the top portion of the cassette are 5.5 x 5.5 mm. The AHC4x24 equires the use of a silicone gasket (Cat. GAHC4x24) to demarcate the individual wells. The GAHC4x24 is included with the purchase of the AHC4x24 product. The top of the cassette is easily sealed with foil seals to prevent evaporation.

Figure 5. Silicone Replacement Gaskets for multi-well microarray Hybridization Cassettes. Use Replacement Gasket GAHC1x16 (upper left) with Hybridization Cassette AHC1x16, Replacement Gasket GAHC1x24 (upper right) with Hybridization Cassette AHC1x24, Replacement Gasket GAHC1x16 four each (lower left) with Hybridization Cassette AHC4x16, and one Replacement Gasket GAHC4x24 (lower right) with Hybridization Cassette AHC4x24. To prevent leakage during use, gaskets must be used in the proper orientation with the wide portion placed against the hybridization cassette top and the thin portion placed against the glass substrate slide. Note: Arrayit offers a variety of gaskets with larger reaction areas.

Figure 6. Schematic drawing for Arrayit Hybridization Cassette 4X8S (Cat. AHC4X8S). AHC4X8S holds 4 glass substrate slides 1 x 25 x 76 mm and gaskets define 8 x 4 wells, with the 8 wells down the slide spaced at 9 mm centers. For larger wells, use Cat. ACH4X8L (see Fig. 7).

Figure 7. Schematic drawing for Arrayit Hybridization Cassette 4X8L (Cat. AHC4X8L). AHC4X8L holds 4 glass substrate slides 1 x 25 x 76 mm and gaskets define 8 x 4 wells, with the 8 wells down the slide spaced at 9 mm centers. For smaller wells, use Cat. ACH4X8L (see Fig. 6).

Figure 8. Schematic drawing for Arrayit Hybridization Cassette 4X24 (Cat. AHC4X24). AHC4X24 holds 4 glass substrate slides 1 x 25 x 76 mm and the gasket defines 24 x 4 wells, with the 24 wells down spaced at 9 mm centers.

Figure 8.  Array Plate Multi-Well Hybridization Stations are available for 4x8, 4x16 and 4x24 Hybridization Cassettes. Click here for product details. Reaction tool easily sealed with foil seals to prevent evaporation during long reactions.  Wash using Microarray Wash Trays and a plate shaker.

Recommended Equipment and Reagents
NanoPrint™ 2 Microarrayers
SpotBot® 4 Personal Microarrayers
InnoScan® Microarray Scanners
Array Plate Multi-Well Hybridization Stations
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuge
Protein Printing Buffer
BlockIt Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers

Ordering Information

Arrayit life sciences products are sold for research purposes only. Arrayit brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any performance issues should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Pricing may vary up to 30% due to costs associated with distribution, import taxes, duties, customs clearance and shipping.