Fluorescent Protein Microarray Platforms
Data Sheet
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Arrayit offers the most advanced and affordable platforms available for complete fluorescent protein microarray experimentation including SpotBot® 3 Protein Edition Personal Microarrayer with cooled platen, SpotLight™ Two-Color Fluorescence Microarray Scanner, BioBlue Bioinformatics Computer, and all of the tools, kits and reagents required to manufacture and deploy protein microarrays. Perfect turnkey solution for antigen, antibody, peptide, reverse phase and carbohydrate microarrays.
Table of Contents
- Introduction
- Platform Components
- Ordering Information
- Warranty
Introduction
Arrayit Fluorescent Protein Microarray Platform empowers customers with an advanced system for micro-scale multiplexed protein microarray assays.
Miniaturizing and multiplexing ELISA based assays reduces cost and increases the accuracy of the assays. When multi analytes are processed in a microarray format, each analyte on the surface of the microarray is subjected to the same exact processing conditions. This reduces variability when multiple samples are analyzed. Additionally the microarray format uses much lower reaction volumes, which increases the concentration of capture agent on the surface to the protein in solution being reacted to the microarray. Higher concentrations mean greater detectivity and higher sensitivity. In addition the microarray format saves money by using 10,000 fold less sample. In the future, the microarray format that our technologies provide will replace all traditional ELISA based 96 well plate and tube-based assays. This powerful set of tools is capable of performing a multitude of other applications including:
- MicroSpot ELISA
- Protein-Protein
- Protein-Drug
- Protein-Antigen
- Protein-Antibody
- Protein-DNA
- Protein-RNA
- Protein-Carbohydrate and more
Figure 1. SpotBot® 4 Protein Edition Personal Microarrayer with cooled platen and chiller bath, enabling desktop protein microarray manufacturing.
Figure 2. SpotLight™ 2 Two-Color Fluorescence Microarray Scanner with Cy3™ and Cy5™ detection and 10 µm scan resolution for desktop fluorescent protein microarray scanning.
Platform Components
The Protein Microarray Platform includes everything you need to perform robust protein microarray experiments. The system includes:
The Protein Edition SpotBot Personal Microarrayer. This unique microarrayer has cold room capabilities built in and delivers sub-nanoliter volumes of sample to protein microarray surfaces using the Stealth Micro Spotting Device. This highly affordable system is capable of printing thousands of samples to 1 microscope slide sized substrate. A 1 year warranty is included in the platform. This platform can also include a NanoPrint microarrayer for higher throughput. Please contact us for a quote.
SpotLight Microarray Scanner. ArrayIt® introduces a new line of microarray scanning instruments that provide improved performance and efficiency compared to commercial laser scanners. SpotLight leverages new “cool” excitation technology, sensitive deep-space imaging detectors, proprietary high numerical aperture lenses and custom filter sets to capture more signal than traditional microarray scanners, while offering greatly reduced background. ArrayIt® sets a new standard in microarray scanning with the 2-color SpotLight Microarray Scanner.
BioBlue Bioinformatics Computer. A powerful desktop PC capable of running all the analysis software and instruments provided with the Platform.
SuperEpoxy 2, SuperNitro and other Protein Microarray Surface Chemistries for high binding and low background when used in conjunction with the SpotLight Microarray Scanner.
Buffers for printing, washing and blocking. Sophisticated blocking solutions formulated to inactive microarray surfaces prior to protein binding and nucleic acid hybridization reactions. Produces ultra-low background allowing greater sensitivity and detection of rare proteins and antibodies, particularly suitable for diagnostic assays. Advanced printing buffer formulated to increase the quality and ease of protein microarray manufacture. Protein printing buffer stabilizes proteins, antibodies, peptides, and other polypeptides by providing sample conditions that mimic the intracellular environment. For use on SuperNitro, SuperAmine, SuperAldehyde, SuperEpoxy and other microarray surfaces.
Reaction Cassettes. The platform includes 3 of these bench tools designed for cover slip microarray reactions suitable for both DNA and protein microarrays.
Reaction Chamber 1x24. The new 24-chamber hybridization cassette fits one microarray substrate (1” x 3” or 25mm x 76mm) and sections it into 24 individual rectangles for up to 24 simultaneous microarray reactions. This hybridization cassette utilizes a compression fit silicone gasket to separate the microarrays, which eliminates the need for contaminating adhesives and inks.
Microarray High Throughput Wash Station. The platform includes 3 bench top microarray-washing tools that cleans 25 substrates/slides on a typical magnetic stir plate.
Microarray Centrifuge. Spin-dries one substrate/slide in seconds after washing.
Figure 3. Arrayit Bioinformatics Mini Computer with the latest commputer hardware and software for high-speed analysis of microarray data in a highly compact computer footprint.
Figure 4. Fluorescent protein microarray scanned at 10 µm scan resolution with the SpotLight™ Two-Color Fluorescence Microarray Scanner. Strong signals and low background permit high detectivity and dynamic range for a wide range of DNA and protein microarray assays.
