Arrayit offers the market’s finest Colorimetric Protein Microarray Platforms, complete desktop and benchtop systems for robust colorimetric experimentation with DNA and protein microarrays.  Platform components  include a SpotBot® 4 Protein Microarrayer or NanoPrint™ Protein Edition Microarrayer with cooled platen and humidity control, a SpotWare™ Colorimetric Microarray Scanner, a Microarray High-Speed Centrifuge, a High-Throughput Wash Station, a Microarray Reaction Cassette, BlockIt™ Blocking Buffer, SuperNitro Microarray Substrate Slides, and an Alkaline Phosphatase Labeling Kit. The Colorimetric Protein Microarray Platforms are highly recommended for research, proteomics, serum profiling and educational purposes.

Table of Contents

• Introduction
• Quality Control
• Product Description
• Platform Contents
• Applications Note
• Technical Assistance
• Complete Protocols
• Troubleshooting Tips
• Recommended Products
• Ordering Information
• Warranty
• Disclaimer

Introduction
Congratulations on taking a important step towards accelerating the speed and versatility of your microarray research. This handbook contains a complete set of protocols detailing the steps required to use the Arrayit Colorimetric Protein Microarray Platforms.

Quality Control
Arrayit ensures the quality and performance of the Colorimetric Protein Microarray Platforms. The most advanced science and technology was used products to develop these products.

Product Description
The Arrayit Colorimetric Protein Microarray Platforms is a comprehensive instruments, hardware, software, reagents, and consummables package for colorimetric protein microarray manufacture and use. Users will appreciate the following system features:

• SpotBot® 4 Protein Edition Microarrayer for desktop protein microarray printing
• NanoPrint™ Protein Edition Microarrayer for benchtop protein microarray printing
• Cooled SpotBot® 4 and NanoPrint™ printing platens maintain protein structure and function
• Humdification and dehumidification allows operation in wide range of ambient conditions
• Arrayit provides world’s most popular personal microarrayers and colorimetric scanners
• SpotWare™ Colorimetric Microarray Scanner enables fast, ultra-sensitive detection
• Adjustable scanning resolution:  5, 10, 20 and 50 µm
• BioBlue Bioinformatics Mini Computer enables high-speed data analysis
• Standard glass substrate slide microarray format: 25 x 76 x 1 mm
• Print 14 microarrays and scan 12 microarrays in a single printing and scanning step
• Computers and flat screens provided with all instruments
• SuperNitro Microarray Substrates are industry’s finest colorimetric microarray surface
• BlockIt™ Blocking Buffer prevents non-specific protein binding
• Alkaline Phosphatase Labeling Kit produces strong signals and low background
• Microarray Hybridization Cassette facilitates protein binding reactions
• Microarray High-Speed Centrifuge rapidly drys substrate slides prior to scanning
• SpotWare™ Scanner produces standard 16-bit TIFF file format for easy analysis
• SpotWare™ adjustable gain function provides an additional 3 logs (1000-fold) sensitivity
• Total dynamic range: > 7 logs (10,000,000-fold)
• Small instruments footprint conserves bench space
• SpotBot® 4 can be used at ambient temperature for colorimetric DNA applications
• Compatible with alkaline phosphatase (AP) and horseradish peroxidase (HRP) labels
• Scan area: 25 x 76 mm with separate barcode detection capability
• Scan speed: 1.0 cm2 in 30 seconds at 10 µm resolution
• User-specified experimental notes attached to each data file
• Total platform set-up time less than 3 hours
• Perfect complement to your existing fluorescent microarray platform
• Reads DNA, RNA, protein, antigen, and antibody microarrays
• Perfect system for both research and diagnostics applications
• Excellent platform for education purposes

Platform Contents
Arrayit Colorimetric Protein Microarray Platform I contains the following components:

• SpotBot® 4 Protein Edition Microarrayer with Megasonic Wash Station
• SpotWare™ Colorimetric Microarray Scanner and Accessories
• BioBlue Bioinformatics Mini Computer with Flat Panel Display
• Microarray High-Speed Centrifuge, 1 each
• High-Throughput Wash Station, 1 each
• Microarray Hybridization Cassette, 1 each
• BlockIt™ Blocking Buffer (250 ml of 1X solution), 1 each
• SuperNitro Microarray Substrate Slides (box of 25), 1 each
• Alkaline Phosphatase Labeling Kit, 1 each
• Complete One-Year Parts Warranty (Microarray Pins Excluded)

Arrayit Colorimetric Protein Microarray Platform II contains the following components:

• NanoPrint™ Enterprise Level Protein Microarrayer with Microplate Cooling
• SpotWare™ Colorimetric Microarray Scanner and Accessories
• BioBlue Bioinformatics Mini Computer with Flat Panel Display
• Microarray High-Speed Centrifuge, 1 each
• High-Throughput Wash Station, 1 each
• Microarray Hybridization Cassette, 1 each
• BlockIt™ Blocking Buffer (250 ml of 1X solution), 1 each
• SuperNitro Microarray Substrate Slides (box of 25), 1 each
• Alkaline Phosphatase Labeling Kit, 1 each
• Complete One-Year Parts Warranty (Microarray Pins Excluded)

Figure 1. The Arrayit Colorimetric Protein Microarray Platform II includes the NanoPrint™ 2 Enterprise Level Protein Edition Microarrayer.

Figure 2. The Arrayit Colorimetric Protein Microarray Platforms includes the SpotWare™ Colorimetric Microarray Scanner.

Figure 3. The Arrayit Colorimetric Protein Microarray Platforms includes a BioBlue Bioinformatics Mini Computer with flat panel display. Please note that computer appearance may differ from the photograph as innovations and upgrades are added to these systems.

Figure 4. The Arrayit Colorimetric Protein Microarray Platforms includes the Microarray High-Speed Centrifuge.

Figure 5. The Arrayit Colorimetric Protein Microarray Platforms includes a protein microarray hybridization cassette.

Figure 6. The Arrayit Colorimetric Protein Microarray Platforms includes a High-Throughput Wash Station.

Figure 7. The Arrayit Colorimetric Protein Microarray Platforms includes SuperNitro Microarray Substrate Slides.

Figure 8. The Arrayit Colorimetric Protein Microarray Platforms includes BlockIt™ Blocking Buffer.

Figure 9. The Arrayit Colorimetric Protein Microarray Platforms includes an Alkaline Phosphatase Labeling Kit.

Applications Note
The Arrayit Colorimetric Protein Microarray Platform empowers users to perform a variety of miniaturized and multiplexed protein microarray assays including microarray-based enzyme-linked immunosorptive assay (ELISA) tests. Miniaturizing and multiplexing ELISA-based assays reduces cost and increases the accuracy of the assays. When multiple analytes are processed in a microarray format, each analyte on the surface of the microarray is subjected to identical reaction and processing conditions.  This reduces variability when multiple samples are analyzed. Additionally, the microarray format uses much lower reaction volumes, which increases the concentration of capture agent on the surface and speeds binding reactions with protein molecules in solution. Greater protein concentrations mean greater detectivity and higher sensitivity. The microarray format offers greater affordability by using 10,000 fold less sample.  The Arrayit microarray format can replace many if not all traditional ELISA-based microplate and tube assays. This powerful set of tools is capable of performing a multitude of other types of assays including:

• Microspot ELISA
• Protein-Protein
• Protein-Drug
• Protein-Antigen
• Protein-Antibody
• Protein-DNA
• Protein-RNA
• Protein-Carbohydrate
• Many others

Visit the protein microarrays website (www.proteinmicroarrays.com) for more information on the types of assays that can be performed with the Arrayit protein microarray platform.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail: arrayit@arrayit.com (under the subject heading, please type ArrayIt technical assistance). 

Complete Protocols
A complete set of installation and technical information is available for each of the products offered in the Arrayit Colorimetric Protein Microarray Platform. Please visit specific web pages for details:

• SpotBot® 4 Protein Edition Microarrayers (upgradeable to NanoPrint™ 2 Protein Edition)
• SpotWare™ Colorimetric Microarray Scanner
• Microarray High-Speed Centrifuge
• High-Throughput Wash Station
• Microarray Hybridization Protein Reaction Cassette
• BlockIt™ Blocking Buffer
• SuperNitro Microarray Substrate Slides
• Alkaline Phosphatase Labeling Kit

Troubleshooting Tips

• Sub-optimal printing quality. Adjust sample volume and wash/dry parameters
• Spots too closely spaced or too far apart in scanned images. Adjust spot spacing parameter in software.
• Uneven background on SuperNitro Microarray Substrates. Increase agitation speed, number of buffer changes and wash times.
• Scanned signals too strong or too weak. Adjust scanner gain settings.
• Scanned spots appear grainy. Increase scanning resolution.

Recommended Products
NanoPrint LM60-2 and LM210-2 Enterprise Level Protein Edition Microarrayers
SpotBot® 4 Protein Edition Personal Microarrayers
SpotWare™ Colorimetric Microarray Scanners
Microarray Printing Pins
Pin Cleaning Kits
SpotBot® Wash Buffer
SuperNitro Microarray Substates
Protein Microarray Buffer Kit
Arrayit 384-well microplates
Arrayit Green and Red Protein Labeling Dyes
Protein Labeling Kit