Products - Microarray Tools - High-Capacity Vacuum Manifold src="images/ Filtration Apparatus for 96-Well and 384-Well DNA Purification Kits

Arrayit High-Capacity Vacuum Manifold (AVM) provides an extremely convenient and high-throughput device for manual and semi-automated vacuum purification of PCR products, dye terminator products, fluorescent probes, and other types of molecules used in microarray analysis.

Table of Contents

• Introduction
• Quality Control
• Product Description
• Technical Assistance
• Short Protocol
• Complete Protocol
• Equipment
• Troubleshooting Tips
• Ordering Information
• Operation Conditions
• Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains information needed to use Arrayit’s ArrayIt® brand High-Capacity Vacuum Manifold (AVM).

Quality Control
Arrayit assures the performance of this product.  The finest scientific research went into the development of this product. Rigorous quality control monitoring of every device guarantees that this vacuum manifold exceeds the highest industry standards.

Product Description
Arrayit’s ArrayIt® brand High-Capacity Vacuum Manifold (AVM) sets a new standard for laboratory vacuum manifolds. The AVM is easy to use, rugged, durable, and can be used with any standard footprint src="images/.

Users will appreciate the following features:

• Compatible with all standard src="images/s
• Accommodates 96- and 384-well plates
• Clear acrylic top allows easy visibility
• Ideal for PCR product, dye terminator and fluorescent probe purification
• Filter 4 plates simultaneously
• Can be operated manually or in a semi-automated manner
• Central reagent tray for easy liquid transfer
• Eluent reservoir holds flow through
• Dual valves for one or two vacuum lines
• Can be used with any vacuum pump
• Small footprint
• Provides years of maintenance-free operation

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail: arrayit@arrayit.com (under the subject heading please type ArrayIt technical assistance).  By telephone: (408) 744-1331, Monday—Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

Short Protocol

1. Remove Vacuum Manifold from packaging material.
2. Connect vacuum spindle to vacuum pump.
3. Place 96-well or 384-well plates into position of 4 filter ports.
4. Pipette samples in filter plates.
5. Turn valve to “on” position to initiate vacuum filtration.
6. Empty eluent reservoir after use.
7. Rinse and wipe dry for storage.

Complete Protocol

1. Remove Vacuum Manifold from packaging material.  Place the Vacuum Manifold on a bench top or other solid working surface.
2. Connect vacuum spindle to vacuum pump. Turn the vacuum valve into the “off” position, such that the red valve is perpendicular to the direction of airflow.  Attach a vacuum pump to the Vacuum Manifold by inserting the vacuum tubing over the threaded vacuum spindle. Power the Vacuum Pump on.
3. Place 96-well or 384-well plates into position of 4 filter ports.  The 4 filter ports on the top of the Vacuum Manifold hold up to 4 standard src="images/s.  The Vacuum Manifold is ideally suited for use with Arrayit’s PCR Purification Kits, Dye Terminator Clean-Up Kits, and Fluorescent Probe Purification Kits.  Empty filter port locations can be fitted with empty plates in experiments using fewer than 4 src="images/s. The Vacuum Manifold is well suited for all vacuum-based filtration procedures.
4. Pipette samples in filter plates.  Use a manual or automatic pipetting device to transfer buffers and samples into the src="images/s. The reagent reservoir on the top center portion of the vacuum manifold can be used to hold buffers during the pipetting steps.
5. Turn valve to “on” position to initiate vacuum filtration. Once the samples have been transferred to the src="images/s, initiate filtration by turning the red vacuum valve one-quarter turn clockwise into the “on” position. Samples should flow slowly through the filter plates and into the eluent reservoir. If a vacuum seal is not achieved, make certain that all 4 src="images/s are seated correctly against the black rubber gaskets in the filter ports.  Vacuum flow can be turned on and off during multiple pipetting steps, by turning the vacuum valve into the open and closed positions. At the end of the filtration process, turn the valve into the “off” position.
6. Empty eluent reservoir after use.  Sample eluent will accumulate in the white eluent reservoir located between the top and bottom portions of the Vacuum Manifold.  Eluent is removed by inverting the reservoir into a sink or waste container.
7. Rinse and wipe dry for storage.  After use, make certain to rinse and clean the Vacuum Manifold in preparation for future use.

Figure 1.  ArrayIt® brand High-Capacity Vacuum Manifold (AVM) shown in disassembled form.  The base, eluent reservoir and clean top fit together into a compact assembly.  Vacuum filtration of PCR products, dye terminator products, fluorescent probes, and other molecules is highly recommended for microarray and DNA sequencing applications.

Equipment and Reagents
Vacuum Pump
PCR Purification Kits
Fluorescent Probe Purification Kits
Dye Terminator Cleanup Kits
Cleanroom Wipes

Troubleshooting Tips
Manifold fails to pull sufficient vacuum

• Make certain a src="images/ is seated in all four src="images/ ports
• Make sure manifold top and base are sealed properly
• Press down firmly on all four src="images/s to initiate the vacuum