Arrayit InnoScan® 710 Series High-Speed High-Resolution Two-Color Microarray Fluorescence Scanners
Data Sheet
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Arrayit offers the InnoScan 710 and 710AL, the world’s latest next generation microarray scanners with greater sensitivity and lower background, proprietary rotary motion control, dynamic auto-focusing, 2-color fluorescence detection, 3 µm scan resolution, 3 minute scan times and complete software. The new infrared 710IR and 710IRAL enable infrared microarray detection.
Product description
- Latest next generation microarray scanning technology
- Fastest and most compact microarray scanners available
- Proprietary rotary scanning architecture permits the fastest scanning speeds
- 3 minutes to scan an entire 25 x 76 mm substrate slide
- Scan 24 substrate slides in 100 minutes using the 710AL
- Adjustable scan resolution from 3 µm to 40 µm
- Ideal for both in situ and ex situ microarrays
- 710 and 710AL detect 532 nm (cyanine 3) and 635 nm (cyanine 5)
- 710IR and 710IRAL detect 670 nm (cyanine 5.5) and 785 nm (infrared dye)
- Compatible IR dyes: IRDye 800 RS (Li-Cor), DyLight 800 (Pierce) and Alexa Fluor 790 (Invitrogen)
- Rotary scanning architecture reduces vibrational noise and increases image quality
- Much greater reliability and longevity than conventional raster scanners
- High resolution acquisition system: scans spot sizes down to 30 µm diameter
- Real-time image acquisition for all microarray types including but not limited to in-situ high density glass slide microarrays, spotted microarrays of all kinds, tissue and cell microarrays
- Low background noise and high sensitivity system
- Uniform scanning across the entire microarray surface regardless of substrate type (glass, membrane and plastic)
- Not sensitive to shocks, vibrations and slide deformation (portable)
- Delivered with Mapix® software: an easy to use data acquisition and image analysis software including automatic spot recognition and powerful image analysis
- Software for various OS environments (Linux, Windows )
- Ethernet interface allows easy data sharing (scanner can be shared over network by several users)
- Affordable price, low maintenance
- Lightweight, robust structure makes it portable system
- Small footprint for laboratory space saving
- Weight: 15 kg
Image acquisition and quantification demo available upon request, email us at arrayit@arrayit.com.
Specifications
Lasers |
Thermoelectrically cooled laser diodes with stabilized feedback output |
Excitation wavelengths |
532 and 635 nm (710 and 710AL); 670 and 785 nm (710IR and 710IRAL) |
Laser power |
Adjustable (2 laser power settings available) |
Gain PMT |
Linear from 0 to 100% |
Autofocus |
Real-time and automated |
Detection |
True confocal and high performances digital laser/PMT system |
Optical resolution |
3 µm |
Pixel size |
3 µm to 40 µm |
Scanning time |
3.5 min per 22 x 74 mm substrate at 10 µm resolution |
Autoloader capacity |
24 standard 25 x 76 mm glass substrate slides |
Autoloader speed |
5 seconds load/unload per substrate slide |
Autoloader throughput |
100 min for 24 substrate slides |
Scan area |
Full substrate slide area (22 x 74 mm) |
Image format |
Standard TIFF (16 bit gray scale) |
Barcode reader |
All formats |
Power supply |
88 - 264 VAC |
Size (L x D x H) |
28 x 46 x 37 cm (model 710); 33 x 60 x 42 cm (model 710AL) |
Weight |
16 Kg (model 710 and 710IR); 27 Kg (model 710AL and 710IRAL) |
Class |
Class I laser product |
Sensitivity |
0.05 fluor/µm² |
Dynamic range |
>10,000-fold |
Uniformity |
<5% CV |
Format |
Open platform glass substrate slides (25 x 76 mm) |
Computer interface |
Ethernet |
Image analysis |
Highly accurate grid positioning using automatic spot recognition provide accurate quantification and 16-bit exportable TIFF data |
Compatible fluorophores: |
Cy5.5 (685 nm); Cy5, Alexa 647, Alexa 660 (635 nm); Cy3, Alexa 546, Alexa 555 (532 nm); 785 nm infrared dyes, and custom wavelengths available |
Figure 1. The ArrayIt® InnoScan® 710 microarray scanners use dynamic auto focusing, which greatly reduces background noise and increases signal intensities. This proprietary auto-focusing technology allows the system to adjust to temperature changes, mechanical perturbations and other system changes that would otherwise cause fluctuations in signal and background. The result is highly uniform scanning across the entire chip surface and consistent performance scan after scan.
Quantitation and analysis software is included with every scanner:
Mapix® is a microarray image analysis software application that is both intuitive and easy to use, combining imaging and analysis tools for superior performance.
Highly efficient algorithms provide rapid and effective feature gridding through automatic spot searching and optimal grid alignment.
- Automatic spot segmentation ensures high-quality experiment data by selecting relevant pixels and eliminating unwanted ones.
- Gridding performance and quantification process are adapted to the high resolution of the InnoScan 710 (3 µm) and therefore to the size of images generated.
- Optimal data visualization and quick learning thanks to its highly interactive commands, with full correlation between displayed data, pictures and plots.
- Compatible all types of microarrays made by ex-situ and in-situ techniques.
- Reads and analyzes interstitial/staggered/orange packing patterned microarrays made by the Protein NanoPrint, Agilent and others.
- Batch processing allows analysis of a large number of microarrays per day to boost productivity (available on Version 3.0.0).
- Regular software updates are accessible on line to match evolving needs of customers (updates are free for the 1st year).
Configurable for full or modular automation of:
- Scanner control
- Configuration saving (scan and analysis parameters)
- Bar code reading
- Quality control by flagging
Compatible with all industry-standard formats and platforms:
- Support GAL files and recordable transcripts in both text and GPR formats.
- Images are saved as 16-bit TIFF images with all read parameters and can be copied in JPEG format.
- Supports image analysis of 16-bit TIFF files from a variety of other scanners.
- Platform-independent and runs on all operating systems (Windows, 32- and 64-bit Linux and Macintosh).
Figure 2. The ArrayIt® InnoScan® 710AL laser microarray scannerwith autoloader provides high resolution, high-speed fluorescence scanning of all standard glass microarrays. The 710AL scans 24 glass substrate slides (25 x 76 mm) in both channels at 10 µm resolution in 100 minutes. The ArrayIt® InnoScan® 710AL laser microarray scanner is the perfect automated solution to high-throughput microarray scanning for research and diagnostics.
