Protein Chips

Services - Protein Chips

Multiplexed Protein Microarray Assay Development Services
Custom protein microarray manufacturing is available from ArrayIt. Our core microarray manufacturing technologies are compatible with all modern microarray detection methods including but not limited to: fluorescence, chemiluminescence, colorimetric, RLS, Planar Waveguides and Mass Spec. The type of assay and detection method will determine what surface chemistry is used to manufacture the microarrays.

Our universal microarray platform empowers us to provide:

Multiplexed Microarray Based ELISA Assays (microspot ELISA)
Antibody Microarrays
Antigen Microarrays
Whole Proteome Microarrays
Reverse Phase Microarrays
Peptide microarrays
and others…

Protein Microarray Applications include but are not limited to:

Expression Profiling
Serum Based Diagnostics
Protein-protein binding assays
Drug-target binding
Receptor epitope binding

Pharmaceutical and biotechnology researchers use protein microarrays to streamline drug target identification, selection, validation, and predictive testing.

Figure 1: Libraries of express proteins are easily printed into microarrays with the NanoPrint Microarrayer. For detection of the printed proteins, samples can be directly labeled or a non-label detection technique can be used. A variety of protein- protein, protein-drug, and protein-small molecule interactions can be implemented. These microarrays can be used to analyze antibody specificity.

Figure 2: Our technologies are suitable miniaturizing and multiplexing assays that use both antigens and antibodies. We can implement MicroSpot ELISA (micro enzyme linked immunosorbent assay) and antibody microarrays. Detection can be fluorescent or colorimetric using secondary antibodies labeled with AP, HRP, biotinylated secondary antibodies that bind labeled streptavidin or direct fluorescent labeling. A variety of sandwich assays can be implemented in multiplexed fashion. As many as 24 identical microarrays can be run per slide.

Figure 3: Our microarray technologies can be used to implement reverse phase microarrays. These microarrays are used to profile bound complex cell lysates, cells captured by LCM or serum samples printed into microarrays. The bound samples can be probed with antibodies for the detection of antigens present in the printed samples. Detection can be accomplished a variety of ways including use of a fluorescently labeled secondary antibody.

Figure 4: Synthetic proteins, peptides and engineered proteins to detect the presence of proteins in complex samples can also be implemented. Unique protein binding events, epitope mapping studies, small molecule binding events or protein/protein interactions can be detected.

Anyone using ArrayIt Services can receive a 60 day free trial of Genesifter.net. www.genesifter.net

Contact: array@arrayit.com or phone 408-744-1331.

Microarray Manufacturing Consulting Services - Get direct and practical “how to” information from the worlds leader of tools, kits and reagents used in microarray manufacturing.