Antibody Microarrays

Products - Printed Microarrays - Antibody Microarrays

Antibody, Antigen, Peptide and other Protein Microarrays Custom Made to Order.
Antibody microarrays to Cancer, Apoptosis, Growth Factors, Cytokines, Receptors and More...
Users can to assay thousands of specific proteins with the ArrayIt Protein Microarrays. A wide variety of proteins representing a range of biological functions are detectable. ArrayIt microarrays allow users to measure differences in protein abundance using a two-color fluorescent labeling technique that can be provided with the microarrays. “Sandwich” detection schemes can also be implemented, please discuss your application goals with us. Detection is typically fluorescent-based using microarray scanners, but microarrays for other detection platforms can be provided. The protocols are simple, and the detection limits are extremely high.

Make your sample selection at:
Proteins are provided by QED BioScience, Inc. http://www.qedbio.com/ QED is preferred because of the special pricing that is implemented to create the microarrays. However, it is possible to manufacture protein microarrays from other sources.

After making the sample selection using QED’s excellent product list, email the list of proteins desired to arrayit@arrayit.com with the subject line reading “custom microarrays”. A quote will be sent to you promptly based on the list provided. These microarrays are affordable. Additionally, you will only pay for microarrays that pass our stringent quality control.

Eamples:
Cancer Antibody Microarray
Antibodies for Cancer Research have become increasingly important tools for the investigation of the progression and regulation of tumor cell development. The cancer antibody microarray contains a diverse panel of monoclonal antibodies for research of tumor cell proliferation, suppression, and drug resistance. These include antibodies to BRCA1, ATM, Mitosin, CA-125, Retinoblastoma (RB) Protein, Topoisomerase II and more...

Apoptosis Antibody Microarray
Programmed cell death or apoptosis is a well-studied cellular phenomenon in multicellular organisms. It is characterized by fragmentation of DNA, plasma condensation, and cell fragmentation followed by formation of membrane vesicles and, finally, clearance of the apoptotic vesicles. A full set of well-characterized antibodies to targets of apoptosis can represented on the microarray.

Growth Factors / Cytokines / Receptor Antibodies
For studies in receptor biology and cytokine functions, well-characterized antibodies to growth factors, cytokines and their receptors are available. These antibodies may be used in immunoassays, histochemistry, or bioassays in a miniaturized and multiplexed microarray format.

Figure 1. Two color antibody microarray. Compare two biological samples to measure the absolute and relative differences in protein expression. This procedure is fluorescence-based and compatible with all current microarray scanners. Extracted proteins are directly labeled (kit provided) and analyzed. The covalently immobilized antibodies capture fluorescently labeled antigens during the reaction step. Normal and cancerous cells can be compared. The raw data provide a measure of proteins from samples A & B (see above graphic).

Nine pAb's with different Ag specificities were spotted in triplicate onto SuperEpoxy surface chemistry using 946MP3 Micro Spotting Pins in a stabilizing protein printing buffer and stored for 2 months at room temperature in the dark. These were assembled in a 96-well microtiter plate format such that a complete set of antibodies is available for multiplex assays per well. Mixtures of biotinylated Ag's were added to each well and allowed to bind to their specific pAbs. The level of specifically bound Ag was quantified by using a streptavidin-Cy3 secondary antibody. In cases where no specific Ag was added for a particular Ab there is no signal detected.