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Arrayit Corporation (ARYC) leads and empowers the genetic, research, pharmaceutical, and diagnostic communities through the discovery, development and manufacture of proprietary life science technologies and consumables for disease prevention, treatment and cure.  - Powerful Science for Life™

Products - Microarrayers - SpotBot® 2 Personal Desktop Microarray Printers

325 installations worldwide make SpotBot® the world’s most successful personal microarray manufacturing instrument.  Via our patented ex-situ technique, SpotBot can configure virtually any biomolecule into a microarray format.

Click here to watch our SpotBot® Movie!

Click here to watch our SpotBot® technical video.

Arrayit-SpotBot2
ArrayIt® offers the finest personal microarrayers available, with more than 300 installations worldwide. Our desktop microarray manufacturing instruments use advanced micro-robotics to ensure precision, compactness and affordability. The SpotBot® 2 Personal Microarrayer will print 384 samples onto 14 substrates in duplicate in 2 hours using four patented 946 Micro Spotting Pins. SpotBot® 2 systems are perfect complements to microarray core facilities, ideal for innovative research and diagnostics, and essential instruments for all microarray laboratories.

Click HERE to view the Standard SpoCLe Generator.
Click HERE to view the Multiple Microarray SpoCLe Generator.
Click HERE to view the Biacore SpoCLe Generator.
Click HERE to view the 96 & 384 Well Plate SpoCle Generator
Humidity Control Apparatus for SpotBot 2.
Megasonic Wash Station Upgrade!
Gal help file: http://www.spotsupport.com/downloads/GALCreatorV2.0.02HELP.pdf

SpotBot® 2 Features and Specifications

  • SpotBot® 2 offers improved speed, precision and durability over SpotBot®
  • 300 installations world-wide
  • Axis resolution and repeatability of ±10 µm
  • Arrayit International patented printing technology (U.S. 6,101,946)
  • 4-pin printhead configuration (2 x 2 at 4.5 mm spacing)
  • Compatible with all Stealth and 946 and Professional Micro Spotting Pins
  • Capacity of 14 standard sized glass substrate slides (25 x 76 mm)
  • Capacity of one 384-well microplate
  • Printing routines allow 1-5 spots per sample
  • 3,600 spots per sub-microarray (9 x 9 mm)
  • 50,400 spots per entire substrate (18 x 63 mm)
  • Printing time of 2 hrs per 384-well microplate
  • >1,000 samples in 6 hours
  • Compatible with Windows XP, 2000, and NT 4.0
  • Power requirements: Standard 120 or 220 volt outlets
  • Ease-of-operation via new version 3 Graphical User Interface (GUI)
  • Size (H x L x W): 22 cm (8.7 in) x 30 cm (11.8 in) x 30 cm (11.8 in)
  • Weight: 6.4 Kg (14 lbs) including vacuum and peristaltic pumps

Unique Features of the SpotBot® 2

  • Only truly portable microarrayer available at 1.0 cubic foot in size.
  • Installation time of less than 60 minutes.
  • Arrives pre-calibrated from our California laboratories.
  • Only system that uses micro-robotics for motion control.
  • “Zero” thermal emission, ultra-low energy consumption, and quiet operation.
  • Uses a forced air stream for pin drying, providing "zero" sample carry-over.
  • Super fast humidity control of 5% per minute and ±1 % at steady state.
  • Ultra-low wash buffer consumption of <1.0 liter per 8 hours of operation.
  • Ultra-low air turbulence minimizes sample evaporation during printing.
  • Ultra-low vibration improves printing quality and eliminates disruption of analytical balances, microscopes, scanners and other sensitive equipment.
  • Private label and OEM packages available to volume customers.
  • Only personal microarrayer in the world.

SpotBot® 2 Components

  • 1 SpotBot® 2 Personal Microarrayer
  • 1 Professional Style 4-Pin Printhead
  • 4 946MP4 Pins (or customer choice of any pin tip size)
  • 1 Calibration Pin (to check factory or customer calibration)
  • 1 Substrate Locator L-Bracket
  • 4 Anti-Vibration Pads (place one under each instrument foot)
  • 1 Air Compressor to power the Dry Station
  • 1 Peristaltic Pump to power the Wash Station
  • Complete Tubing Set (installed at factory)
  • 1 Wash Buffer Reservoir (1 liter capacity)
  • 1 Waste Buffer Container (1 liter capacity)
  • 1 Liter of 1X SpotBot® 2 Wash Buffer
  • 1 x 50 ml of 2X Micro Spotting Solution Plus
  • 1 x 25 SuperAmine Microarray Substrates (for test printing)
  • 1 x 384-Well Microplate
  • 1 Voltage Converter (110-220 volt)
  • 1 COM Port Data Transfer Cable
  • 1 SpotSuite Software Installation CD
  • 1 Calibration CD (must be installed by the customer prior to operation!)
  • Full One-Year Parts and Warranty Package (extra charge)
  • (Option) SpotBot® 2 Complete Desktop Manufacturing System
  • (Option) SpotBot® 2 Protein Edition with Cooled Platen
  • (Option) Humidity Control Apparatus
  • (Option) Megasonic Wash Station

Important Technical Note
SpotBot® 2 Personal Microarrayers are calibrated in our laboratories in California to ensure proper Z-axis height over the substrates and platen, and correct pin placement in the wash and dry station, and 384-well microplate. These calibration files are provided on a compact disc in addition to the SpotSuite 3 installer CD. Calibration files are unique to your system and must be installed prior to operating your SpotBot® 2 system!

SpotBot-circut-board
Figure 1.
SpotBot® 2 printed circuit board. Shown is the printed circuit board and operation lights of the SpotBot® 2. Operation is computer-controlled and fully automated.  The printhead shown in this photograph contains a calibration pin in the first of 4 pin apertures.

microarray-printer-pins
Figure 2.
Shown is the wash and dry station of the SpotBot® 2, with the wash port (left) and the drying port (right). The printhead containing 4 Stealth Pins is fully inserted into the dry station in this photograph such that the collars rise above the top surface of the printhead by about 1.0 mm. Customers can also use 946 Pins with extended collars for ease-of-handling and greater printing precision. A forced air stream generated by the SpotBot® 2 Air Compressor dries each pin independently, essentially eliminating sample carry-over. Cross-contamination can be further reduced further with the Megasonic Wash Station option, a highly recommended option for exacting users and protein microarray applications.

desktop-personal-microarrayer
Figure 3.
Shown is the SpotBot® 2 in the sample loading position. After the wash/dry step, the 4-pin Printhead moves over the first loading position in the 384-well microplate, lowers to the bottom of the plate, and pauses for 3 seconds allowing the samples load into the pin tips by capillary action. Each pin loads 0.2 µl of sample using the SMP4 or 946MP4 pin design. The recommended sample volume is 5-10 µl per well in a 384-well microplate. The SpotBot® 2 will print an entire 384-well plate across 14 substrates in less than 2 hours.

spotbot-microarrayer
Figure 4.
Microarray printed with dilutions of ArrayIt® Green540 and Red640 fluorescent dyes using the SpotBot® 2 equipped with four 946MP4 Pins and a 946PH4 Printhead. All samples were printed in quintuplicate at 200-µm center-to-center spacing on SuperAmine Substrates. The uniformity of the rows and columns of printed spots reveals the excellent printing performance of the SpotBot® 2. The space bar denotes 500 µm in the image.

Arrayit-SpotBot
Figure 5.
  Multiple microarrays per substrate printed with a SpotBot® 2 Personal Microarray Robot. Shown is a photograph of the SpotBot® 2 platen containing SuperAmine Microarray Substrates printed with a SpotBot® 2 Personal Microarrayer running 946MP4 Pins printing at 175 µm spacing. A total of 24 microarrays per substrate were printed using the Multiple Microarray Format SpoCLe Generator version 1.1.02, which can be downloaded at http://www.spotsupport.com.

SPOCLE
Figure 6.
  Multiple Microarray Format SpoCLe Generator version 1.1.02 allows the printing of multiple microarrays per substrate with SpotBot 2 Personal Microarrayers (see Fig. 5). Shown is a screenshot of the software interface. The user selects the number of microarrays or “well pattern”, spot spacing in microns, print offsets in millimeters, and subgrid dimensions as columns and rows. The software then generates a graphic showing how the printed microarrays (orange icons) will appear on the substrates. The Multiple Microarray Format SpoCLe Generator version 1.1.02 can be downloaded at
www.spotsupport.com.

microarray-printer-pump
Figure 7. Shown is a photograph of the Peristaltic Pump used on SpotBot®2 Personal Microarrayers. Tubing colors should be configured as shown. The speed dial should be set to the right (CW, clockwise setting) for correct buffer flow into the Wash Station. Pumps are either 12 rpm or 100 rpm as designated on the back of the instrument under “speed”.  A proper buffer flow rate of 1.0 ml/min into the SpotBot®2 Wash Station is achieved using dial setting 8 (12 rpm pump) or dial setting 1 (100 rpm pump, shown here). Tubing connections (not shown) should be green-green, blue-blue, yellow-yellow, and white-white as designated on the tubing ends and hardware:  green-green = Peristaltic Pump intake to Wash Buffer Reservoir; blue-blue = Peristaltic Pump intake to Wash Station outlet; white-white = Peristaltic Pump outlet to Wash Station intake; yellow-yellow = Peristaltic Pump outlet to Wash Waste Container.

Arrayit-desktop-microarrayer
Figure 8. Shown is a photograph of the SpotBot®2 Personal Microarrayer including the accessories. The translucent wash buffer reservoir, orange air compressor for the dry station, silver peristaltic pump for the wash station, silver megasonic power supply and humidification system including the orange air compressor, humidification reservoir and gray valve are shown correctly configured behind the instrument. The modular and compact accessories improve system utility and performance and simplify maintenance and repair. Inside the instrument, the microplate, L-bracket and temperature/humidity pen are visible.

microarray-printer-sonic
Figure 9. Shown is a photograph of the SpotBot®2 Personal Microarrayer including the accessories. The translucent wash buffer reservoir, orange air compressor for the dry station, silver peristaltic pump for the wash station, silver megasonic power supply and humidification system including the orange air compressor and humidification reservoir are shown correctly configured behind the instrument. The COM cable, power supply, dry station fitting (red), wash buffer fitting (white), waste buffer outtake (blue), and humidification tubing are shown correctly attached to the back of the instrument. The modular and compact accessories design improves system utility and performance and simplifies maintenance and repair.

microplate-printing
Figure 10. SpotBot®2 Microplate Printing. SpotBot®2 Personal Microarrayers enable microarray printing into 96-well microplates (shown) and 384-well microplates, as well as onto microplate-sized glass substrates using the standard SpotBot®2 components and Well Plate Printing Spocle Generator v2.0.2 software running on SpotApp v3.5.7. Microplate printing software is downloadable on the SpotSupport web site. Microplate printing requires the Microplate L-Bracket Locator (Cat. MLS) to hold the microplate in position during printing, and utilizes two pre-print substrates as shown here. System calibration is required prior to printing. Please do not attempt microplate printing prior to system calibration.

Scientific Publications
Click on the links to review recent scientific publications featuring ArrayIt® brand SpotBot® Personal Microarrayers.

Recommended Equipment and Reagents
NanoPrint™ 2 Enterprise Level Microarrayers
Microarray Contact Printing Technology
InnoScan® Microarray Laser Scanners
SpotLight™ Personal Microarray Scanners
SpotWare™ Colorimetric Microarray Scanners
Microarray High-Speed Centrifuge
High-Throughput Wash Stations
Microarray Hybridization Cassettes
Super Microarray Substrates
Microarray Purification Kits
Microarray Hybridization and Blocking Buffers
Microarray Print Buffers
Microarray Wash Buffers
SpotBot® 2 Wash Buffer

Ordering Information

Catalog ID

Description

Price (US dollars)*

SPA2

SpotBot® 2 Personal Microarray System with version 3 software and intuitive graphical interface. Improved durability, precision, usability and performance compared to our original SpotBot®. Uses Arrayit’s patented printing technology (U.S. 6,101,946). Complete 22-component system brings microarray manufacturing to the desktop. The world’s only personal microarrayer just got better with SpotBot® 2. Ideal for research and diagnostics, a perfect complement to large microarrayers, and essential for every microarray laboratory.

The newest version of this product is SPA3

UG2S

Software and hardware upgrade to convert your SpotBot® into a SpotBot® 2. Upgrade includes disassembly, cleaning, refurbishing, and extensive software and hardware upgrade for all SpotBot® Systems including SpotBot®, SpotBot® Complete, and SpotBot® Protein Edition.  The Upgrade restores and improves performance and extends SpotBot® longevity at a fraction of the cost of a new SpotBot® 2.

The newest version of this product is UG3S

PWS

Complete one-year parts and service warranty for the SpotBot® 2 Personal Microarray System. Warranty coverage includes all system components used in a regular manner except pins and printheads. Warranty does not cover system damage caused by irregular use.

$2,888

PWS2

Complete two-year parts and service warranty for the SpotBot® 2 Personal Microarray System. Warranty coverage includes all system components used in a regular manner except pins and printheads. Warranty does not cover system damage caused by irregular use.

$4,794

PWS3

Complete three-year parts and service warranty for the SpotBot® 2 Personal Microarray System. Warranty coverage includes all system components used in a regular manner except pins and printheads. Warranty does not cover system damage caused by irregular use.

$6,064

NWS

Non-warranty repair for all SpotBot® 2 Personal Microarrayers, including system inspection, testing, diagnosis, disassembly, parts, re-assembly, testing, calibration, cleaning, and printhead and wash station cleaning. Cost to replace platens, accessories and instrument cases may be higher. Please allow 3-6 weeks for non-warranty repairs.

$8,432

HCSPA

Humidity Control Apparatus for SpotBot® 2 Personal Microarray Systems SPA2, SPA2XP, and SPA2PRO, available in 110 volt and 220 volt systems.

$1,606

DSPA

Dehumidification Apparatus for all SpotBot® and SpotBot® PRO 1, 2 and 3 Personal Microarray Systems, reduces humidity from ambient to <10% RH ±1%, includes humidity control compressor, dessicator tube, control valve, air filter, hygrometer, and tubing, available in 110 volt and 220 volt systems.

$1,629

MSW110V

Megasonic Wash Station for SpotBot® 2 Personal Microarray Systems SPA2, SPA2XP, and SPA2PRO, 110 volt domestic version.

$3,292

MSW220V

Megasonic Wash Station for SpotBot® 2 Personal Microarray Systems SPA2, SPA2XP, and SPA2PRO, 220 volt overseas version.

$3,350

SPH4

4-Pin Stealth Printhead for SpotBot® 2 Personal Microarray Systems.

$745

946PH4

4-Pin 946 Printhead for SpotBot® 2 Personal Microarray Systems.

$780

ACS

Air Compressor for SpotBot® 2 Personal Microarray Systems.

$1,109

PPS

Peristaltic Pump for SpotBot® 2 Personal Microarray Systems.

$1,825

WDS

Wash and Dry Station for SpotBot® 2 Personal Microarray Systems.

$1,473

SLS

Substrate Locator for SpotBot® 2 Personal Microarray Systems.

$249

MLS

Microplate L-Bracket Locator to Position Microplates for Microplate Printing on SpotBot® 2 Personal Microarray Systems.

$322

CTS

Complete tubing set for SpotBot® 2 Personal Microarray Systems.

$531

WBS

Wash Buffer for SpotBot® 2 Personal Microarray Systems, sterile and free of particulate and biological contaminants, ideal for all microarray wash stations including sonic systems, shipped pre-made and ready to use, 1 liter of 1X solution.

$91

HFS

Humidity fitting for SpotBot® and SpotBot® 2 Personal Microarray Systems, 2 3/16” long with double 1/4” hose barbs, retainer nut and rubber humidity gasket. Connects to humidification and de-humidification options.

$87

AIC

Arrayit High-Performance Instrument Cleaner removes dust, particulates, fingerprints and other contaminants from SpotBot® 2 microarrayers. Apply using pump nozzle and Cleanroom Wipes, 65 ml of 1X solution.

$110

WBD

Wash Buffer Dispenser Bottle for SpotBot® 2 Personal Microarray Systems, 1 liter capacity.

$149

WWC

Waste Wash Container for SpotBot® 2 Personal Microarray Systems, 1 liter capacity.

$149

*For the most current pricing information, technical assistance, and shipping schedules please contact Arrayit by telephone 408-744-1331, FAX 408-7441711, email arrayit@arrayit.com, or click on the purchase buttons above to proceed directly to the purchase page.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

*Add shipping and handling to all orders.

 

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