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Products - Microarrayers - SpotBot® 2 - Megasonic Wash Station to Enhance Microarray Printing Pin Cleaning in SpotBot® 2 Desktop Personal Microarrayer Wash Stations

microarray-megasonic
Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Installation
  • Recommended Products
  • Ordering Information
  • Warranty

Introduction
Congratulations on taking a big step towards improving your microarray research. This booklet contains complete protocols outlining the steps required to use the SpotBot Megasonic Wash Station.

Quality Control
Arrayit assures the performance of these products, and the finest scientific research went into their development.

Product Description
The SpotBot® Megasonic Wash Station increases the performance of the SpotBot® wash station through the use of megasonic waves (>400,000 Hz) provided by a piezoelectric transducer located in the base of the wash station.  Instead of circulating the wash buffer passively through the wash station, the Micro Spotting Pins are cleaned with wash buffer in the presence of high frequency ultrasonic (Megasonic) power.  This SpotBot® upgrade is very easy to install and use, and is superior to cleaning systems that rely on more traditional ultrasonic (25-100,000 Hz) cleaning which is more harsh and less efficient.  The Pin cleaning mechanism relies on cavitation and turbulent liquid flow as shown in Figures 1 and 2.

microarray-pin-sonic
Figure 1. Megasonic waves are produced with a piezoelectric transducer located in the base of the wash station.  The transducer is driven by a modular Power Supply, which provides a continuous source of alternating current (AC) power input.  The ceramic transducer (rectangle), optimized for geometry and other the physical properties, is excited at its resonance frequency, producing acoustic waves (white circles) and a large number of cavitation bubbles (white spheres) that exert highly efficient cleaning on the microarray Pins (gray).  Acoustic waves (white arrows) are also propagated through the wash buffer (blue) providing additional cleaning by turbulent liquid flow. The combination of cavitation and turbulent flow remove sample from the sample channel of the Pin and clean the Pin surface by removing sub-micron sample molecules (red spheres) adhering to the Pin surfaces.  Megasonic waves, which travel at a higher frequency than ultrasonic waves, clean more gently than ultrasonics, eliminating microscopic surface pitting and other forms of surface wear that can occur with some ultrasonic cleaning systems.

microarray-printer-ultrasonic
Figure 2. Megasonic waves are produced, causing high-frequency cavitation and turbulence in the wash buffer of the SpotBot® Wash Station.  Pins immersed in megasonic-driven wash buffer are cleaned in a highly efficient manner, essentially eliminating sample carry-over and pin clogging, even when particulate-containing or “sticky” samples are used for printing. The Wash Station must be filled with wash buffer at all times or serious damage can occur to the megasonic transducer and wash station. Please remember to power off the megasonic power supply after each use!

microarray-spotter-wash-station
Figure 3A. Data showing 5 blank buffer spots spotted, followed by 5 Cy3 labeled oligo spots, followed by 5 blank buffer spots using the SpotBot® microarrayer and SMP4 Micro Spotting Pins. There is no detectable carryover following the dye labeled spots.

microarray_washstation_software
Figure 3B. Default wash/dry programming settings used to perform the study.  Data collected on an Axon 4000B microarray scanner.

Installation (retrofitting an existing SpotBot® system)
Disconnect all of the hardware from the standard SpotBot® wash station including the peristaltic pump, dry station compressor, and fittings. Remove the standard wash station from the platen by unscrewing (counterclockwise) the four recessed attachment screws located on the top left and right sides of the wash station (see Fig. 2).  Re-attach the wash station hardware to the Megasonic Wash Station, place it on the platen, and attach it to the platen using the 4 attachment screws by turning the screws clockwise.  Re-connect the peristaltic pump and air compressor to the Megasonic Wash Station.  Connect the power cord and transducer cable to the back of the Megasonic Driver as shown in Figure 3 to complete the hardware installation. Do not power on the Megasonic Power Supply at this step! The Wash Station must be filled with wash buffer at all times or serious damage can occur to the megasonic transducer and wash station!

microarray-megasonic-cleaning
Figure 4. The connections on the back of the Megasonic Wash Station Power Supply should look like this. Please do not power on this unit unless the Wash Station is filled with Wash Buffer. The Power Supply should be turned off after each printing session.

Proceed to the software installation steps at this point.  Run SpotApp from the tools menu to select the Wash and Dry Module Calibration procedure and click “Yes” as shown in the graphic below.

spotbot-wash-station
This first step is required to calibrate the SpotBot® to the top of the Wash station.  Insert the test pin into the SpotBot® printhead and use the computer mouse to click the lateral and vertical tabs in the Wash Fountain – Upper window (see graphic below) to move the test pin so that the tip of the test pin makes minimalcontact with the top of the wash station.

spotbot-calibration
This calibration procedure sets the SpotBot® “safe” Z-axis height so that pins move freely over the top of the wash station during operation. Click “Done” when the proper Z-axis height is achieved.

The next step is to calibrate the Z-axis height required for pin washing and drying. The Megasonic Wash Station does not have a contact pad like the standard SpotBot® wash station, so calibration is performed using a slightly different procedure. Use the computer mouse to click the lateral and vertical tabs in the Wash Fountain – Lower window (see graphic below) to move the test pin so that the tip of the test pin is 2 millimeter (2 mm) below the top surface of the wash station. This will provide a 2-3 mm deep layer of wash buffer for pin cleaning, due to the formation of a meniscus in the wash station reservoir. Do not calibrate the pin against the bottom surface of the wash station, which contains the megasonic transducer.  Direct contact between the Pins and the bottom of the wash station will result in permanent pin damage!Click “Done” when the correct position 2 mm below the top of the wash station is obtained.

Arrayit-calibration
The next step is to calibrate the SpotBot® safe Z-axis height for the dry station. Using the computer mouse and test pin, click on the lateral and vertical tabs in the Dry Station – Upper window (see graphic below) to move the test pin so that the tip makes minimalcontact with the top surface of the wash station. This calibrates the SpotBot® so that the pins to move safely above the top surface of the dry station during the pin cleaning process. Click “Done” when the proper location is achieved.

microarray-spotter-calibration
The final step is to calibrate the SpotBot® height for proper pin drying.  The dry station has a contact pad inside the dry chamber for easy calibration. Using the computer mouse and test pin, click on the lateral and vertical tabs in the Dry Station – Lower window (see below) to move the test pin so that it goes completely into one of the holes of the dry station and makes minimal contact with the bottom of the dry station. Minimal contact is achieved when the test Pin very slightly raises out of the Printhead, most easily observed as a slight space between the bottom of the pin collar and the printhead. Click “Done” when the correct position is obtained.  This completes the calibration procedure and the Megasonic Wash Station is now ready to use.

microarray-calibration-procedure

Warning: Never operate the Megasonic Wash Station unless the wash station is completely filled with wash buffer, and wash buffer is flowing freely from the wash buffer reservoir to the waste container. Attempting to operate the Megasonic Wash Station in the absence of liquid can cause permanent damage to the piezoelectric crystal.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading, please type ArrayIt technical assistance). By telephone: (408) 744-1331, Monday–Friday PST 8:30am - 5:30 PM.  Please remember that we want to hear about your successes!

Recommended Products

Ordering Information

Product

Description

Catalog ID

Price (US dollars)*

Megasonic Wash Station for SpotBot® Microarrayer Systems SPA, SPAXP, and SPAPRO; 110 volt domestic version

Modular enhancement providing megasonic pin cleaning to reduce pin clogging and sample cross-contamination for the most exacting printing applications

MSW110V

$3,374

Megasonic Wash Station for SpotBot® Microarrayer Systems SPA, SPAXP, and SPAPRO; 220 volt overseas version

Modular enhancement providing megasonic pin cleaning to reduce pin clogging and sample cross-contamination for the most exacting printing applications

MSW220V

$3,434

SpotBot® Wash Buffer

Wash Buffer for the SpotBot®, 1X solution in 1 liter bottles, sterile and free of particulate and biological contaminants, ideal for all microarray wash stations including sonic systems, shipped pre-made and ready to use, 1 liter of 1X solution.

WBS

$93

*For the most current pricing information, technical assistance, and shipping schedules please contact arrayit@arrayit.com Phone: 408-744-1331.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

*To order ArrayIt® Brand Products: call 408-744-1331, fax 408-744-1711 or click on the purchase button to proceed directly to the purchase page.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes only. ArrayIt® products are not for use in human diagnostics or for drug purposes, nor are they to be administered to humans in any way. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

 

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