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Products - Microarrayers - SpotBot® 2 - Humidity Control Apparatus to Regulate SpotBot® 2 Desktop Personal Microarrayer Relative Humidity Levels Inside the Instrument

microarray-printer-humidity
Arrayit's SpotBot® 2 Personal Microarray Robot provides the world’s first desktop/laptop microarray printing system at an affordable price. The addition of the new Humidification System allows the user to control the environment inside the unit between ambient and 50% above ambient relative humidity with +- 1 % accuracy. An added benefit of using the humidification device is the filtration of particles from the supply air. After two hours of humidification/filtration, 0.5 um particles inside the SpotBot® 2 chamber measured <1,700 ppm and 0.7 um particles inside the SpotBot® 2 chamber measured <600 ppm. Ambient readings were 5002 and 2200 respectively.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Set-Up Protocol
  • Troubleshooting

Introduction
Congratulations on taking a big step towards improving the affordability, quality and speed of your genomic, proteomic, biomedical, pharmaceutical and agricultural research.  This handbook contains all the information required to take full advantage of Arrayit's ArrayIt® brand SpotBot® Humidity Control Apparatus.  Whether you’re a student or a seasoned researcher, SpotBot® will add tremendous value to your scientific endeavors.

Quality Control
Arrayit takes every measure to assure the quality of our ArrayIt® brand SpotBot® Personal Microarray products.  The finest manufacturing procedures were used to manufacture these systems. Rigorous quality control monitoring guarantees that each system conforms to the highest industry standards.

Product Description
Arrayit's ArrayIt®  brand SpotBot® Humidification System provides high quality environmental control for Arrayit’s ArrayIt SpotBot® Personal Microarray Robot. This unit could also be used to add humidity to enclosed environments similar in size.  Precise environmental control is an important factor in high quality microarray manufacturing. The performance and price of this personal microarray humidification system provides the hands-down best value in the industry.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance by electronic mail at arrayit@arrayit.com (subject heading "SpotBot technical assistance"). By telephone: (408) 744-1331, Monday–Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

Set-Up Protocol

  • Parts List
  • a) Pump/needle valve/tubing/air diffuser assembly
  • b) 1000 ml Erlenmeyer Flask/tubing assembly
  • c) Digital hygrometer

The pump/needle valve/tubing assembly and flask/tubing assembly have been partially assembled prior to shipping to ensure easy and efficient set-up.

  • Step 1
  • Fill the 1000 ml Erlenmeyer flask to the 900 ml mark with distilled water.
  • Step 2
  • Insert green air diffuser into the flask and fit the black stopper snuggly into the flask opening. The flask is now assembled to the orange air pump.
  • Step 3
  • Attach the loose end of the tubing extending from the flask arm to the single white barb on the rear of the SpotBot®. The apparatus is now completely assembled.

II. Printing with Humidification
Prior to starting the print run it is advisable to raise and stabilize the humidity inside the SpotBot® to the desired level.

  • Step 1
  • Insert the substrates onto the SpotBot® platen and secure them using the Substrate Locator.
  • Step 2
  • Place the supplied hygrometer inside the SpotBot® chamber just inside the front door placed parallel to the door in front of the substrates and substrate locator bar with the display facing the ceiling. Close all SpotBot® doors.

IMPORTANT NOTE: Proper placement of the hygrometer is critical since an improperly placed device may interfere with SpotBot® Printhead or mechanics and may result in serious damage to the unit.

  • Step 3
  • Plug the air pump into a power supply and flip on the power switch. You should immediately observe air bubbling through the water inside the flask and an increase in humidity inside the SpotBot® chamber.
  • Step 4
  • Adjust the airflow through the flask by tightening or loosening the needle valve regulating knob. To raise humidity quickly, open the needle valve completely. This will raise the humidity approximately 1% every 20 seconds. Once the desired level is attached, adjust the needle value down to maintain the desired level. Dryer conditions will require the valve to be more open. Humidity can be controlled within one percent of the desired level.
  • Step 5
  • When the desired level has been stabilized, insert the first microplate and begin your print run.

Note: Some humidity is likely to be lost when the SpotBot® door is opened. After closing the door it may make a few minutes to stabilize the humidity to the desired level.

Troubleshooting Tips
1. The pump has been running for 5 minutes or longer and no change is reflected on the hygrometer display.
No power to pump.  Make sure pump is attached to working power source and switch is in the ON position.
Needle valve is closed. Loosen needle valve knob by turning to the left.
Tubing is clogged. Methodically disconnect tubing at each joint and check for airflow.
Hygrometer is faulty. Remove hygrometer from SpotBot® chamber and check power by turn power off and on. Hold in reset button until you see display without min or max designation.
2. Humidity in SpotBot® chamber is too low.
Too little airflow through system. Loosen the needle valve regulating knob by turning knob to the left until desired humidity level is reached.
Tubing is crimped. Check full length of tubing for crimping and straighten all tubing being sure weight of tubing is not causing restricted flow.
3. Humidity in SpotBot® chamber is too high.
Too much airflow through system. Tighten the needle valve regulating knob by turning knob to the right or turn off pump and open SpotBot® doors until humidity is sufficiently reduced.
4. Humidity level is oscillating too high and too low.
Needle valve regulating knob is being adjusted too severely. When adjusting the airflow, turn knob in small increments or decrements and allow 3-5 minutes between adjustments for stabilization.

desktop-microarray-printer
Figure 1. Shown is a photograph of the SpotBot®2 Personal Microarrayer including the accessories. The translucent wash buffer reservoir, orange air compressor for the dry station, silver peristaltic pump for the wash station, silver megasonic power supply and humidification system including the orange air compressor, humidification reservoir and gray valve are shown correctly configured behind the instrument. The modular and compact accessories improve system utility and performance and simplify maintenance and repair. Inside the instrument, the microplate, L-bracket and temperature/humidity pen are visible.

microarray-printer-megasonic
Figure 2. Shown is a photograph of the SpotBot®2 Personal Microarrayer including the accessories. The translucent wash buffer reservoir, orange air compressor for the dry station, silver peristaltic pump for the wash station, silver megasonic power supply and humidification system including the orange air compressor and humidification reservoir are shown correctly configured behind the instrument. The COM cable, power supply, dry station fitting (red), wash buffer fitting (white), waste buffer outtake (blue), and humidification tubing are shown correctly attached to the back of the instrument. The modular and compact accessories design improves system utility and performance and simplifies maintenance and repair.

Scientific Publications
Click on the links to review recent scientific publications featuring ArrayIt® brand SpotBot® Personal Microarrayers.

Recommended Equipment and Reagents
NanoPrint™ 2 Enterprise Level Microarrayers
Microarray Contact Printing Technology
InnoScan® Microarray Laser Scanners
SpotLight™ Personal Microarray Scanners
SpotWare™ Colorimetric Microarray Scanners
Microarray High-Speed Centrifuge
High-Throughput Wash Stations
Microarray Hybridization Cassettes
H25K Whole Human Genome Microarrays
Super Microarray Substrates
Microarray Purification Kits
Microarray Hybridization Buffers
Microarray Blocking Buffers
Microarray Print Buffers
Microarray Wash Buffers
SpotBot® 2 Wash Buffer

Ordering Information

Product

Catalog ID

Price (U.S. Dollars)*

Humidity Control Apparatus for all SpotBot® 1, 2 and 3 Personal Microarrayers containing an Air Compressor, Humidity Chamber, Control Value, Tubing and Hygrometer allowing humidity control from ambient to 80% RH ±1%, highly recommended for all SpotBot® systems to reduce sample evaporation and improve printed spot morphology and coupling efficiency, available in 110 and 220 volt configurations.

HCSPA

$1,606

Dehumidification Apparatus for all SpotBot® 1, 2 and 3 Personal Microarrayers containing an Air Compressor, Dessicator Tube, Control Value, Tubing and Hygrometer, reduces humidity from ambient to <10% RH ±1%, particularly recommended for all SpotBot® Protein systems to prevent moisture buildup during cooling, available in 110 and 220 volt configurations.

DSPA

$1,629

MegaSonic Wash Station for all SpotBot® 2 Personal Microarrayers containing a Power Supply, Megasonic Wash Station for highly efficient megasonic cleaning of microarray printing pins, highly recommended for all SpotBot® 2 systems to improve pin cleaning and reduce sample carry-over, 110 volt domestic version.

MSW110V

$3,292

MegaSonic Wash Station for all SpotBot® 2 Personal Microarrayers containing a Power Supply, Megasonic Wash Station for highly efficient megasonic cleaning of microarray printing pins, highly recommended for all SpotBot® 2 systems to improve pin cleaning and reduce sample carry-over, 220 volt overseas version.

MSW220V

$3,350

For the most current pricing information, technical assistance, and shipping schedules please contact us by email arrayit@arrayit.com, telephone 408-744-1331, or FAX 408-744-1711.

To order ArrayIt® Brand Products: call 408-744-1331, fax 408-744-1711 or click on the purchase buttons to proceed directly to the product page.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

*Plus shipping and handling.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

 

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