Products - Microarray Tools - Multi-Well Microarray Hybridization Cassettes to Multiplex Multiple Samples and Microarray Tests on Glass Microarray Substrate Slides

Arrayit offers innovative hardware solutions for creating multi-well microarrays on a single glass substrate slide. Reaction cassettes containing 8, 16, 24, 48 or 96 wells utilize silicone gaskets to create individual reaction wells encompassing the printed microarrays. The upper portion of the Hybridization Cassette allows the introduction of individual samples, making these cassettes ideal for DNA and protein microarray research and diagnostics.

Figure 2. Diagramatic depiction of the wells formed by Hybridization Cassette 1x24 (left) and 1x16 (right). Use these spacing coordinates to program the microarrayer prior to printing the 3x8 (1x24) or 2x8 (1x16) microarray patterns required to utilize the multi-well Hybridization Cassettes. Use the SpotBot and NanoPrint Microarrayer to simplify the microarray manufacturing using these formats. NOTE! It is possible to cut the ribs in these gaskets to make larger size reaction areas!

Figure 3. Hybridization Cassette 4x16 (Catalog ID AHC4X16) is identical to Hybridization Cassette 1x16 except that it contains four identical units allowing the use of four glass substrate slide microarrays. The Hybridization Cassette 4x16 has a 7.5 x 7.5 mm well size set at 9 mm centers in four identical 2x8 units. Well openings in the top portion of the Hybridization Cassette (shown here) are 5.5 x 5.5 mm. Requires the use of four individual silicon gaskets (Catalog ID GAHC1x16) to demarcate the individual wells, and the four gaskets are included with the purchase of the AHC4x16 product. Easily sealed with foil seals to prevent evaporation.

Figure 4. Hybridization Cassette 4x24 (Catalog ID AHC4X24) is identical to Hybridization Cassette 1x24 except that it contains four identical units allowing the use of four glass substrate slide microarrays. The Hybridization Cassette 4x24 has a 7.5 x 6.5 mm well size set at 9 mm centers in four identical 3x8 units. Well openings in the top portion of the Hybridization Cassette (shown here) are 5.5 x 5.5 mm. Requires the use of a single silicone gasket (Catalog ID GAHC1x24) to demarcate the individual wells, and one GAHC1x24 gasket is included with the purchase of the AHC4x24 product. Easily sealed with foil seals to prevent evaporation.

Figure 5. Silicone Replacement Gaskets for multi-well microarray Hybridization Cassettes. Use Replacement Gasket GAHC1x16 (upper left) with Hybridization Cassette AHC1x16, Replacement Gasket GAHC1x24 (upper right) with Hybridization Cassette AHC1x24, Replacement Gasket GAHC1x16 four each (lower left) with Hybridization Cassette AHC4x16, and one Replacement Gasket GAHC4x24 (lower right) with Hybridization Cassette AHC4x24. To prevent leakage during use, gaskets must be used in the proper orientation with the wide portion placed against the hybridization cassette top and the thin portion placed against the glass substrate slide. NOTE! It is possible to cut the ribs in these gaskets to make larger size reaction areas!

Figure 6. Schematic drawing for Arrayit Hybridization Cassette 4X8S (Cat. AHC4X8S). AHC4X8S holds 4 glass substrate slides 1 x 25 x 76 mm and gaskets define 8 x 4 wells, with the 8 wells down the slide spaced at 9 mm centers. For larger wells, use Cat. ACH4X8L (see Fig. 7).

Figure 7. Schematic drawing for Arrayit Hybridization Cassette 4X8L (Cat. AHC4X8L). AHC4X8L holds 4 glass substrate slides 1 x 25 x 76 mm and gaskets define 8 x 4 wells, with the 8 wells down the slide spaced at 9 mm centers. For smaller wells, use Cat. ACH4X8L (see Fig. 6).

Figure 8. Schematic drawing for Arrayit Hybridization Cassette 4X24 (Cat. AHC4X24). AHC4X24 holds 4 glass substrate slides 1 x 25 x 76 mm and the gasket defines 24 x 4 wells, with the 24 wells down spaced at 9 mm centers.

Figure 8.  Array Plate Multi-Well Hybridization Stations are available for 4x8, 4x16 and 4x24 Hybridization Cassettes. Click here for product details. Reaction tool easily sealed with foil seals to prevent evaporation during long reactions.  Wash using Microarray Wash Trays and a Plate Shaker.

Recommended Equipment and Reagents
NanoPrint™ 2 Microarrayers
SpotBot® 4 Personal Microarrayers
InnoScan® Microarray Scanners
Array Plate Multi-Well Hybridization Stations
Microarray Hybridization Cassettes
High Throughput Wash Station
Microarray High-Speed Centrifuge
Protein Printing Buffer
BlockIt Blocking Buffer
Microarray Air Jet
Microarray Cleanroom Wipes
PCR Purification Kits
Micro-Total RNA Extraction Kit
MiniAmp mRNA Amplification Kit
Indirect Amino Allyl Fluorescent Labeling Kit
Universal Reference mRNA
Green540 and Red640 Reactive Fluorescent Dyes
Hybridization Buffers

Ordering Information

Product	Description	Catalog ID	Price (US Dollars)*
Hybridization Cassette, 1x16	ArrayIt® multi-well Hybridization Cassette containing a base, 16-well silicon gasket and a top portion, 16 7.5 x 7.5 mm wells in a 2x8 pattern at 9 mm centers, single glass substrate slide microarray capacity.	AHC1X16	$369
Hybridization Cassette, 1x24	ArrayIt® multi-well Hybridization Cassette containing a base, 24-well silicon gasket and a top portion, 24 7.5 x 6.5 mm wells in a 3x8 pattern at 9 mm centers, single glass substrate slide microarray capacity.	AHC1X24	$369
Hybridization Cassette, 4x16	ArrayIt® multi-well Hybridization Cassette containing a base, four 16-well silicon gaskets and a top portion, 64 7.5 x 7.5 mm wells in four 2x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.	AHC4X16	$572
Hybridization Cassette, 4x24	ArrayIt® multi-well Hybridization Cassette containing a base, four 24-well silicon gaskets and a top portion, 96 7.5 x 6.5 mm wells in four 3x8 patterns at 9 mm centers, four glass substrate slide microarray capacity.	AHC4X24	$572
Hybridization Cassette, 4x8 Small Wells	ArrayIt® multi-well Hybridization Cassette containing a base, 4x8-well silicon gaskets and a top portion, 32 1 x 8 x 4 wells 17 mm wide, 100-200 µl well volume at 9 mm centers, 4 glass substrate slide microarray capacity.	AHC4X8S	$572
Hybridization Cassette, 4x8 Large Wells	ArrayIt® multi-well Hybridization Cassette containing a base, 4x8-well silicon gaskets and a top portion, 32 1 x 8 x 4 wells 23 mm wide, 150-300 µl well volume at 9 mm centers, 4 glass substrate slide microarray capacity.	AHC4X8L	$572
Replacement Gaskets GAHC1x16	Silicon Replacement Gasket for the ArrayIt® multi-well Hybridization Cassettes AHC1x16 and AHC4x16, allowing the formation of 16 7.5 x 7.5 mm wells in a 2x8 pattern at 9 mm centers. Use 1 each for AHC1x16 and four each for AHC4x16.	GAHC1X16	$144
Replacement Gaskets AHC1x24	Silicon Replacement Gasket for the ArrayIt® multi-well Hybridization Cassette AHC1x24, allowing the formation of 24 7.5 x 6.5 mm wells in a 3x8 pattern at 9 mm centers.	GAHC1X24	$144
Replacement Gaskets 4x16	Silicon Replacement Gaskets for the Arrayit multi-well Hybridization Cassette AHC1x16, 4 gaskets per set.	GAHC4X16	$329
Replacement Gaskets 4x24	Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.	GAHC4X24	$329
Replacement Gaskets 4x8S	Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.	GAHC4X8S	$329
Replacement Gaskets 4x8L	Silicon Replacement Gasket for the Arrayit multi-well Hybridization Cassette AHC4x24, 1 gasket each.	GAHC4X8L	$329
Microarray Wash Tray	Convenient wash tray to use with multi-well hybridization cassettes.	MWT	$193
Microarray Hybridization Station	Convenient instrument to control temperature and mix with these hybridization multi-well hybridization cassettes	MMHS110V and MMHS220V	link to page.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

*To order ArrayIt® brand products: call 408-744-1331, fax 408-744-1711, email or click on the Purchase buttons to proceed directly to the Shop Site.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with any ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

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