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Products - Microarray Platforms - Colorimetric Protein Microarray Platforms

protein_microarrayer
Arrayit offers the market’s finest Colorimetric Protein Microarray Platforms, complete desktop and benchtop systems for robust colorimetric experimentation with DNA and protein microarrays.  Platform components  include a SpotBot® 3 Protein Microarrayer or NanoPrint™ Protein Edition Microarrayer with cooled platen and humidity control, a SpotWare™ Colorimetric Microarray Scanner, a Microarray High-Speed Centrifuge, a High-Throughput Wash Station, a Microarray Reaction Cassette, BlockIt™ Blocking Buffer, SuperNitro Microarray Substrate Slides, and an Alkaline Phosphatase Labeling Kit. The Colorimetric Protein Microarray Platforms are highly recommended for research, proteomics, serum profiling and educational purposes.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Platform Contents
  • Applications Note
  • Technical Assistance
  • Complete Protocols
  • Troubleshooting Tips
  • Recommended Products
  • Ordering Information
  • Warranty
  • Disclaimer

Introduction
Congratulations on taking a important step towards accelerating the speed and versatility of your microarray research. This handbook contains a complete set of protocols detailing the steps required to use the Arrayit Colorimetric Protein Microarray Platforms.

Quality Control
Arrayit ensures the quality and performance of the Colorimetric Protein Microarray Platforms. The most advanced science and technology was used products to develop these products.

Product Description
The Arrayit Colorimetric Protein Microarray Platforms is a comprehensive instruments, hardware, software, reagents, and consummables package for colorimetric protein microarray manufacture and use. Users will appreciate the following system features:

  • SpotBot® 3 Protein Edition Microarrayer for desktop protein microarray printing
  • NanoPrint™ Protein Edition Microarrayer for benchtop protein microarray printing
  • Cooled SpotBot® 3 and NanoPrint printing platens maintain protein structure and function
  • Humdification and dehumidification allows operation in wide range of ambient conditions
  • Arrayit provides world’s most popular personal microarrayers and colorimetric scanners
  • SpotWare™ Colorimetric Microarray Scanner enables fast, ultra-sensitive detection
  • Adjustable scanning resolution:  5, 10, 20 and 50 µm
  • BioBlue Bioinformatics Mini Computer enables high-speed data analysis
  • Standard glass substrate slide microarray format: 25 x 76 x 1 mm
  • Print 14 microarrays and scan 12 microarrays in a single printing and scanning step
  • Computers and flat screens provided with all instruments
  • SuperNitro Microarray Substrates are industry’s finest colorimetric microarray surface
  • BlockIt™ Blocking Buffer prevents non-specific protein binding
  • Alkaline Phosphatase Labeling Kit produces strong signals and low background
  • Microarray Hybridization Cassette facilitates protein binding reactions
  • Microarray High-Speed Centrifuge rapidly drys substrate slides prior to scanning
  • SpotWare™ Scanner produces standard 16-bit TIFF file format for easy analysis
  • SpotWare™ adjustable gain function provides an additional 3 logs (1000-fold) sensitivity
  • Total dynamic range: > 7 logs (10,000,000-fold)
  • Small instruments footprint conserves bench space
  • SpotBot® 3 can be used at ambient temperature for colorimetric DNA applications
  • Compatible with alkaline phosphatase (AP) and horseradish peroxidase (HRP) labels
  • Scan area: 25 x 76 mm with separate barcode detection capability
  • Scan speed: 1.0 cm2 in 30 seconds at 10 µm resolution
  • User-specified experimental notes attached to each data file
  • Total platform set-up time less than 3 hours
  • Perfect complement to your existing fluorescent microarray platform
  • Reads DNA, RNA, protein, antigen, and antibody microarrays
  • Perfect system for both research and diagnostics applications
  • Excellent platform for education purposes

Platform Contents
Arrayit Colorimetric Protein Microarray Platform I contains the following components:

  • SpotBot® 3 Protein Edition Microarrayer with Megasonic Wash Station
  • SpotWare™ Colorimetric Microarray Scanner and Accessories
  • BioBlue Bioinformatics Mini Computer with Flat Panel Display
  • Microarray High-Speed Centrifuge, 1 each
  • High-Throughput Wash Station, 1 each
  • Microarray Hybridization Cassette, 1 each
  • BlockIt™ Blocking Buffer (250 ml of 1X solution), 1 each
  • SuperNitro Microarray Substrate Slides (box of 25), 1 each
  • Alkaline Phosphatase Labeling Kit, 1 each
  • Complete One-Year Parts Warranty (Microarray Pins Excluded)

Arrayit Colorimetric Protein Microarray Platform II contains the following components:

  • NanoPrint™ Enterprise Level Protein Microarrayer with Microplate Cooling
  • SpotWare™ Colorimetric Microarray Scanner and Accessories
  • BioBlue Bioinformatics Mini Computer with Flat Panel Display
  • Microarray High-Speed Centrifuge, 1 each
  • High-Throughput Wash Station, 1 each
  • Microarray Hybridization Cassette, 1 each
  • BlockIt™ Blocking Buffer (250 ml of 1X solution), 1 each
  • SuperNitro Microarray Substrate Slides (box of 25), 1 each
  • Alkaline Phosphatase Labeling Kit, 1 each
  • Complete One-Year Parts Warranty (Microarray Pins Excluded)

microarrayer
Figure 1. The Arrayit Colorimetric Protein Microarray Platform II includes the NanoPrint™ 2 Enterprise Level Protein Edition Microarrayer.

microarray_colorimetric_scanner
Figure 2. The Arrayit Colorimetric Protein Microarray Platforms includes the SpotWare™ Colorimetric Microarray Scanner.

microarray_bioblue_mini_computer
Figure 3. The Arrayit Colorimetric Protein Microarray Platforms includes a BioBlue Bioinformatics Mini Computer with flat panel display. Please note that computer appearance may differ from the photograph as innovations and upgrades are added to these systems.

microarray_high_speed_centrifuge
Figure 4. The Arrayit Colorimetric Protein Microarray Platforms includes the Microarray High-Speed Centrifuge.

microarray_hybridization_cassette
Figure 5. The Arrayit Colorimetric Protein Microarray Platforms includes a protein microarray hybridization cassette.

microarray_wash_station
Figure 6. The Arrayit Colorimetric Protein Microarray Platforms includes a High-Throughput Wash Station.

microarray_nitrocellulose_substrate_slide
Figure 7. The Arrayit Colorimetric Protein Microarray Platforms includes SuperNitro Microarray Substrate Slides.

microarray_blocking_buffer
Figure 8. The Arrayit Colorimetric Protein Microarray Platforms includes BlockIt™ Blocking Buffer.

microarray_alkaline_phosphatase
Figure 9. The Arrayit Colorimetric Protein Microarray Platforms includes an Alkaline Phosphatase Labeling Kit.

Applications Note
The Arrayit Colorimetric Protein Microarray Platform empowers users to perform a variety of miniaturized and multiplexed protein microarray assays including microarray-based enzyme-linked immunosorptive assay (ELISA) tests. Miniaturizing and multiplexing ELISA-based assays reduces cost and increases the accuracy of the assays. When multiple analytes are processed in a microarray format, each analyte on the surface of the microarray is subjected to identical reaction and processing conditions.  This reduces variability when multiple samples are analyzed. Additionally, the microarray format uses much lower reaction volumes, which increases the concentration of capture agent on the surface and speeds binding reactions with protein molecules in solution. Greater protein concentrations mean greater detectivity and higher sensitivity. The microarray format offers greater affordability by using 10,000 fold less sample.  The Arrayit microarray format can replace many if not all traditional ELISA-based microplate and tube assays. This powerful set of tools is capable of performing a multitude of other types of assays including:

  • Microspot ELISA
  • Protein-Protein
  • Protein-Drug
  • Protein-Antigen
  • Protein-Antibody
  • Protein-DNA
  • Protein-RNA
  • Protein-Carbohydrate
  • Many others

Visit the protein microarrays website (www.proteinmicroarrays.com) for more information on the types of assays that can be performed with the Arrayit protein microarray platform.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail: arrayit@arrayit.com (under the subject heading, please type ArrayIt technical assistance).  By telephone: (408) 744-1331, Monday–Friday PST 8:30am - 5:30 PM.  Please remember that we want to hear about your successes!

Complete Protocols
A complete set of installation and technical information is available for each of the products offered in the Arrayit Colorimetric Protein Microarray Platform. Please visit specific web pages for details:

Troubleshooting Tips

  • Sub-optimal printing quality. Adjust sample volume and wash/dry parameters
  • Spots too closely spaced or too far apart in scanned images. Adjust spot spacing parameter in software.
  • Uneven background on SuperNitro Microarray Substrates. Increase agitation speed, number of buffer changes and wash times.
  • Scanned signals too strong or too weak. Adjust scanner gain settings.
  • Scanned spots appear grainy. Increase scanning resolution.

Recommended Products
NanoPrint LM60-2 and LM210-2 Enterprise Level Protein Edition Microarrayers
SpotBot® 4 Protein Edition Personal Microarrayers
SpotWare™ Colorimetric Microarray Scanners
Microarray Printing Pins
Pin Cleaning Kits
SpotBot® Wash Buffer
SuperNitro Microarray Substates
Protein Microarray Buffer Kit
Arrayit 384-well microplates
Arrayit Green and Red Protein Labeling Dyes
Protein Labeling Kit

Ordering Information

Product

Description

Catalog ID

Price (U.S. dollars)*

Colorimetric Protein Microarray Platform I

Arrayit’s Colorimetric Protein Microarray Platform I is a complete desktop system that enables robust colorimetric protein microarray experimentation.  Platform components include a SpotBot® 4 Protein Microarrayer with cooled platen and dehumidification and humidification control and megasonic wash stationl, SpotWare™ Colorimetric Microarray Scanner, BioBlue Bioinformatics Mini Computer, Microarray High-Speed Centrifuge, High-Throughput Wash Station, Microarray Hybridization Cassette, BlockIt™ Blocking Buffer (250 ml of 1X), SuperNitro Microarray Substrate Slides (Box of 25), and Alkaline Phosphatase Labeling Kit. All platform components (excluding microarray pins) covered by one-year warranty for normal use.

CPMPV1

$47,355

Colorimetric Protein Microarray Platform II

Arrayit’s Colorimetric Protein Microarray Platform II is a complete benchtop system that enables robust colorimetric protein microarray experimentation.  Platform components  include a NanoPrint 2 Enterprise Level Protein Microarrayer with microplate cooling dehumidification and humidification control and megasonic wash stationl, SpotWare™ Colorimetric Microarray Scanner, BioBlue Bioinformatics Mini Computer, Microarray High-Speed Centrifuge, High-Throughput Wash Station, Microarray Hybridization Cassette, BlockIt™ Blocking Buffer (250 ml of 1X), SuperNitro Microarray Substrate Slides (Box of 25), and Alkaline Phosphatase Labeling Kit. All platform components (excluding microarray pins) covered by one-year warranty for normal use.

CPMPV2

$213,675

To place an order call 408-744-1331, fax 408-744-1711, email arrayit@arrayit.com, or click on the purchase button above to proceed directly to the purchase page.

*International orders may vary by 35% or more due to import fees, duties, technical support and other costs.

*Plus shipping and handling.

Warranty
ArrayIt® Brand products have been scientifically developed and are sold for research purposes. ArrayIt® Brand products are not for use in human diagnostics or for drug purposes, nor are they to be administered to humans in any way.  Extreme care and exact attention should be practiced in the use of the materials described herein.  All ArrayIt® Brand products are subject to extensive quality control and are guaranteed to perform as described when used properly.  Any problems with any ArrayIt® Brand product should be reported to Arrayit immediately.  Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product including the use of the wrong protocols or procedures is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.

Disclaimer
Arrayit® SpotWare™ Colorimetric Microarray Scanners are not designed or licensed for use with United States Patent 7,321,829 and foreign counterparts held by Eppendorf Biochip Systems GmbH. Arrayit Corporation expressly forbids the use of SpotWare™ for infringing applications and disclaims any liability resulting from improper use of this product.

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