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Products - Microarray Instruments - Ozone Free Boxes
Arrayit Ozone Free Boxes and Accessories create optimal working environments for microarrays that use fluorescent dyes shown to degrade in ozone levels above 5 parts per billion (5 ppb). All ambient air contains ozone and the level of ozone can vary from 10 to more than 124 ppb depending on environmental factors. Laboratory and other equipment such as common laser printers and computers also generate ozone. Shown here is an Innoscan scanner, TrayMix Hybrdization Station and Microarray Centrifuge installed inside an ozone and dust free work enclosure. Ozone-free levels and cleanliness are maintained by an air curtain created across the front of the enclosure.
The system removes ozone and dust from the ambient air, to prevent the destruction of fluorescent dyes and keeps microarrays and equipment clean. Problems with specific dyes commonly used microarrays are reported by the following publications:
Effects of Atmospheric Ozone on Microarray Data Quality.
Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements.
http://www.genomics.princeton.edu/dunham/ozone.html
QA/QC: challenges and pitfalls facing the microarray community and regulatory agencies
Protocols for the assurance of microarray data quality and process control.
Recent advances of protein microarrays
How it Works: The Ozone Free Box operates by using an air circulation loop which forces the air inside the modular enclosure through a highly efficient catalytic converter, converting ozone to oxygen. Dust and small particles are also removed by an additional hepa filter located at the entry of the centrifuge fan. The incorporation of the most powerful catalytic monolith technology guarantees an ozone level lower than 1 ppb with the door closed and 5 ppb with the door open. Under normal atmospheric and temperature conditions, the catalyst filter should last two years. Ozone is converted with an airflow of only 1.5 m/s.
Figure 2. From an ambient concentration of 23 ppb, ozone levels in the Ozone Free Box are reduced to lower than 1 ppb in less than 3 minutes. Moreover, even when the door of the Ozone Free Box is open, ozone levels generally do not exceed 5 ppb. An open door is necessary to allow end users to perform common microarray tasks such as processing and scanning microarrays.
The enclosure is provided as a set of sealed anodized aluminum profiles with PMMA panels that are fastened together to form a workspace adaptable to the desired dimensions for any laboratory environment. The entire front of the enclosure act as either vertical or horizontal doors which open and close very easily. Each enclosure we deliver is custom built to accommodate any size equipment. Please advise us of your requirements and we will prepare a quotation. email arrayit@arrayit.com
Figure 3. Comparison of identical microarrays exposed to different levels of ozone prior to scanning. Improved signal to noise ratios are easily observed. Cy5 is a registered trademark of GE Healthcare.
Figure 4. The enclosure is provided as a set of sealed anodized aluminum profiles with PMMA panels that are fastened together to form a work space adaptable to the desired dimensions for any laboratory environment. The size of the enclosure can be adjusted to accommodate any size equipment. The entire front of the enclosure can act as either vertical or horizontal doors which open and close very easily.
A. B.  C. 
Figure 5. Shown are Ozone Free Box Accessories including the (A) Ozone Sensor Head (A) B. Front Display Panel C. Filter Change
Specifications
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Dimensions and Work Area
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Ozone Free Boxes are available in several convenient work area configurations to meet the needs of all microarray applications. The depth is 80 cm for all models, the height is 80 cm or 100 cm, and length is 100 cm, 150 cm or 200 cm. Please add 21 cm to the actual length of each system for the Catalyst Filter Unit (shown in blue in Figs 4 and 5). See Ordering Information for enclosure dimensions.
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Box and Catalytic Converting Unit
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Anodized Aluminum Profiles with Transparent and Blue PMMA
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Doors
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PMMA plate with handles either vertical or horizontal according to the user needs
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Catalytic Monolith
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Dimensions
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125 x 125mm
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Thickness
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15mm
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Type
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Aluminum filter using MnO2
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Air Flow
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1.5 m/s
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High Precision Sensor
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Mounted on support inside the enclosure connection to display via RJ45 connection
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Accuracy
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+- 0.1 parts per billion
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Calibrated Range
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0.000 – 0.150 ppm
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Response Time
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Less than 70 seconds
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Operating Temperature
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-5 to 50 degrees Celsius
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Power Supply
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12 VDC, 800 mA
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Fan
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1650 tr/mn – 150 W Adjustable speed, dust and particle filter
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Digital Display
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Realtime monitoring of ozone levels with a programmable alarm
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Electrical Requirements
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Power 105 W, Voltage 220V
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Switch
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ON/OFF located in the front of the unit
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Ordering Information
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Product
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Description
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Catalog ID
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Price (US dollars)*
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Ozone-Free Box, 80 cm x 100 cm
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Ozone-Free Box, 80 cm height x 100 cm length x 80 cm depth, ozone and dust free working area, plus 22.5 cm of exterior space for the control unit and catalyzer filter box. Recommended for all microarray applications requiring a low ozone environment.
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OFB80
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$17,850
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Ozone-Free Box, 100 cm x 100 cm
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Ozone-Free Box, 100 cm height x 100 cm length x 80 cm depth, ozone and dust free working area, plus 22.5 cm of exterior space for the control unit and catalyzer filter box. Recommended for all microarray applications requiring a low ozone environment.
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OFB100
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$18,900
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Ozone-Free Box, 80 cm x 150 cm
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Ozone-Free Box, 80 cm height x 150 cm length x 80 cm depth, ozone and dust free working area, plus 22.5 cm of exterior space for the control unit and catalyzer filter box. Recommended for all microarray applications requiring a low ozone environment.
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OFB150
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$19,425
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Ozone-Free Box, 100 cm x 200 cm
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Ozone-Free Box for Agilent Scanners, 100 cm height x 200 cm length x 80 cm depth, ozone and dust free working area, plus 22.5 cm of exterior space for the control unit and catalyzer filter box. Recommended for all microarray applications requiring a low ozone environment.
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OFB200
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$23,100
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Ozone-Free Box Maintenance Kit
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Ozone-Free Box Maintenance Kit including two ozone replacement catalyzer filters and one ozone sensor head, compatible With Ozone Free Box Cat. ID OFB80, OFB100, OFB150 and OFB200.
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OFBMK
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$1,523
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Ozone Sensor Head
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Ozone sensor head, 0 to 0.50 ppm O3, replace every 6-12 months depending on use, compatible with Cat. OFB80, OFB100, OFB150 and OFB200. Recommended for all microarray applications requiring a low ozone environment.
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BTZ
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$1,008
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Replacement Calalyzer Filter
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Replacement catalyzer filter, replace every 6-12 months depending on use, 12.5 x 1.5 x 12.5 cm, for use with Cat. OFB80, OFB100, OFB150 and OFB200. Recommended for all microarray applications requiring a low ozone environment.
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CCF
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$368
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Ozone Free Box Cleaning Kit
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Ozone-Free Box Cleaning Kit including a telescopic mop and 150 wipes, compatible With Ozone Free Box Cat. ID OFB80, OFB100, OFB150 and OFB200.
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OFBCK
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$305
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To order ArrayIt® brand products: call 408-744-1331, fax 408-744-1711, email arrayit@arrayit.com, or click on the purchase buttons above to proceed directly to the purchase page.
*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.
*Add shipping and handling to all orders.
Warranty
ArrayIt® brand products are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with these products should be reported to Arrayit immediately. Our liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.
Copyright 1993-2011 Arrayit Corporation. All rights reserved.
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