Products - Microarray Instruments - SpotBot® 2 - Multiple SPOCLE

Multiple Microarray Format SpoCLe (SpotBot Control Language) software is designed to use the MMH4X24, MMH4X16, AHC1X24 and AHC1X16 Hybridization Chambers and Cassettes. These printing formats are also compatible with other slide-based platforms that allow for multiple microarrays on a single 25 x 76 x 1mm (1” x 3”) slides.  For more information on these products please see:
http://www.arrayit.com/Products/Substrates/Microplate_Microarrays/microplate_microarrays.html
and
http://www.arrayit.com/Products/MicroarrayH/AHC1X24/ahc1x24.html

Screen Shot 1: Start with the factory default to start a new program, or select an existing SpoCLe file to edit.  Click Next.

Screen Shot 2: Select your pin type form the drop down menu.  This will provide a suggested center-to-center spot spacing later in the software. This software interface uses 1 Pin, since the locations on the slides will be at 9mm spacing and the Pin spacing on the SpotBot Printhead is at 4.5 mm spacing.

Screen Shot 3: The software can accommodate up to 1 full 384 well plate.  By selecting “Partial Microplate”.  The configuration of partial plates is designated using the computer mouse to denote the wells that contain samples, as shown by the green and red shading. The last sample and last printed sample is red.  Enter the number of spot replicates for each well desired into the dialog box “Spots Per Sample”.  Selecting 3 Spots Per Sample, for example, would print each sample in triplicate at the spot spacing defined by the user (see next page). The Micro Spotting Pin can be reloaded after a user-defined amount of spots with one load. Washing and drying micro spotting pins is optional by clicking the box Wash/Dry Pin between reloading.

Screen Shot 4A.

Screen Shot 4B.

Screen Shot 4C.

Screen Shot 4D.

Screen Shot 4E,  4 A-E Description: Select well pattern, spot spacing, lateral and vertical start and stop position of the microarrays in each “well”, and define the numbers of columns and row for the microarray. Each microarray in each well will be identical.

Screen Shot 5: The SpotBot holds 14 slides or substrates of 25 x 76 x 1mm (1” x 3”).  By double clicking on a location a slide can be printed, unused for used as a pre-print slide.  Pre-printing is used to remove excess sample from a Micro Spotting Pin if the volume in the source plate is too high.  See catalog ID MMP384 on the arrayit.com website for more information.

Screen Shot 6: Use these dialog boxes to adjust the pin resting or “dwell” time during sample loading, pre-printing and printing. The dwell time is corresponds to the length of time the pins rest on a particular surface measured in seconds.  Specify each value in the “Dwell Times” dialog boxes for “Sample Loading”, “Pre-printing” and “Printing”.  The “Sample Loading” dwell time refers to the length of time the pins rest on the bottom of the 384-well microplate. The “Pre-printing” and “Printing” dwell times denote the length of time the pins “pause” on the printing substrates between the down and upstroke. The factory defaults are 3.0 sec, 0 sec and 0 sec, respectively for the three settings.  The default dwell times produce efficient sample loading and printing in most cases. Viscous samples may require a longer dwell time for efficient loading. A 50 msec (0.05 sec) dwell time for “Printing” can be used to produce larger spots or to increase printing efficiency on highly hydrophobic substrates.

Screen Shot 7: The recommended settings are shown in the “Settings for printing pin cleansing routines” dialog boxes above.  A minimum of 4 Wash/dry cycles is recommended to remove samples from the previous printing cycle.  Using fewer than 4 Wash/Dry cycles may result in sample “carry over” into the subsequent printing cycle.  For most applications, an extended Wash/Dry cycle (2.0 sec and 10.0 sec) is recommended for the final Wash/Dry step to ensure that residual sample has been removed and that the pins are dry for the subsequent sample-loading step.  The parameters governing the final Wash/Dry cycle are specified by checking the “Different Durations for Last Cycle” dialog box. The first 3 Wash/Dry cycles are kept brief (0.5 sec) to prevent sample drying on the pins.

Screen Shot 8: Use these dialog boxes to save the SPOCLE file onto the computer hard drive. The default name of SPOCLE files created by the SPOCLE Generator includes a date and time stamp.  To change the file name and/or path of the file, click on the “save as” to the “SPOCLE Microarray Printing File Path” drop-down menu, and enter the user-specified information. To generate a content map file, check the Content Map” box.  The content map file will be saved in the same folder as the “SPOCLE Microarray Printing File Path”, and will provide the location of each sample printed on the substrates.  This file can also be used to generate a GAL file using the SpotBot GAL file creator program found at www.myspotbot.com.  Content maps are comma-delimited text files that can be imported into spreadsheets and database programs.  SPOCLE files (*.spo) that are stored on the computer hard drive can be opened and run with the SpotApp program.

Catalog ID Description Quantity Price (U.S. dollars)* SPA2 SpotBot® 2 Personal Microarray System with version 3 software and intuitive graphical interface. Improved durability, precision, usability and performance compared to our original SpotBot®.  Uses TeleChem’s patented printing technology (U.S. 6,101,946). Complete 22-component system brings microarray manufacturing to the desktop.  The world’s only and best personal microarrayer just got better with SpotBot® 2. Ideal for basic research and R&D, and a perfect complement to large microarrayers. 1 each $17,050 UG2S Software and hardware upgrade to convert your SpotBot® into a SpotBot® 2.  Upgrade includes disassembly, cleaning, refurbishing, and extensive software and hardware upgrade for all SpotBot® Systems including SpotBot®, SpotBot® Complete, and SpotBot® Protein Edition.  The Upgrade restores and improves performance and extends SpotBot® longevity at a fraction of the cost of a new SpotBot® 2. 1 each $3,988 PWS Full one-year parts and warranty package for the SpotBot® 2 Personal Microarray System (excludes Printhead and Pins) 1 each $2,200 MSW110V Megasonic Wash Station for SpotBot® 2 Personal Microarray Systems SPA2, SPA2XP, and SPA2PRO, 110 volt domestic version 1 each $2,640 MSW220V Megasonic Wash Station for SpotBot® 2 Personal Microarray Systems SPA2, SPA2XP, and SPA2PRO, 220 volt overseas version 1 each $2,805 SPH4 4-Pin Stealth Printhead for SpotBot® 2 Personal Microarray Systems 1 each $545 946PH4 4-Pin 946 Printhead for SpotBot® 2 Personal Microarray Systems 1 each $575 ACH Air Compressor for SpotBot® 2 Personal Microarray Systems 1 each $840 PPS Peristaltic Pump for SpotBot® 2 Personal Microarray Systems 1 each $1,210 WDS Wash and Dry Station for SpotBot® 2 Personal Microarray Systems 1 each $1,085 SLS Substrate Locator for SpotBot® 2 Personal Microarray Systems 1 each $115 CTS Complete tubing set for SpotBot® 2 Personal Microarray Systems 1 each $355 WBS Wash Buffer for SpotBot® 2 Personal Microarray Systems, 1X solution in 1 liter bottles, sterile and free of particulate and biological contaminants, ideal for all microarray wash stations including sonic systems, shipped pre-made and ready to use 1 liter $49 WBD Wash Buffer Dispenser Bottle for SpotBot® 2 Personal Microarray Systems, 1 liter capacity 1 each $19 WWC Waste Wash Container for SpotBot® 2 Personal Microarray Systems, 1 liter capacity 1 each $19

*For the most current pricing information, technical assistance, and shipping schedules please contact the ArrayIt® Division: Phone 408-744-1331, email: arrayit@arrayit.com.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

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