Ozone Free Box

Products - Microarray Instruments - Ozone and Dust Free Work Environment

The Ozone Free Box is intended to create an optimal working environment for microarrays that use fluorescent dyes shown to degrade in ozone levels above 5 parts per billion(ppb). All ambient air contains ozone: The level of ozone in the air can vary from 10 to more than 124 ppb, depending on environmental factors. Additionally, laboratory and other equipment such as laser printers and computers also generate ozone.

Figure 1. Innoscan 700, TrayMix 2 Hybridization Station and Microarray Centrifuge installed inside an ozone and dust free work enclosure. Ozone and cleanliness are maintained with the door open by an air curtain.

The system removes ozone and dust from the ambient air, to prevent the destruction of fluorescent dyes and keeps microarrays and equipment clean. Problems with specific dyes commonly used microarrays are reported by the following publications:

Effects of Atmospheric Ozone on Microarray Data Quality.

Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements.

http://www.genomics.princeton.edu/dunham/ozone.html

QA/QC: challenges and pitfalls facing the microarray community and regulatory agencies

Protocols for the assurance of microarray data quality and process control.

Recent advances of protein microarrays

How it Works: The Ozone Free Box operates by using an air circulation loop which forces the air inside the modular enclosure through a highly efficient catalytic converter, converting ozone to oxygen. Dust and small particles are also removed by an additional hepa filter located at the entry of the centrifuge fan. The incorporation of the most powerful catalytic monolith technology guarantees an ozone level lower than 1 ppb with the door closed and 5 ppb with the door open. Under normal atmospheric and temperature conditions, the catalyst filter should last two years. Ozone is converted with an airflow of only 1.5 m/s.

Figure 2. From an ambient concentration of 23 ppb, ozone levels in the Ozone Free Box are reduced to lower than 1 ppb in less than 3 minutes. Moreover, even when the door of the Ozone Free Box is open, ozone levels generally do not exceed 5 ppb. An open door is necessary to allow end users to perform common microarray tasks such as processing and scanning microarrays.

The enclosure is provided as a set of sealed anodized aluminum profiles with PMMA panels that are fastened together to form a workspace adaptable to the desired dimensions for any laboratory environment. The entire front of the enclosure act as either vertical or horizontal doors which open and close very easily. Each enclosure we deliver is custom built to accommodate any size equipment, please advise us of your requirements and we will prepare a quotation (email; arrayit@arrayit.com ).

Figure 3. Comparison of identical microarrays exposed to different levels of ozone prior to scanning. Improved signal to noise ratios are easily observed. Cy5 is a registered trademark of GE Healthcare.

Figure 4. The enclosure is provided as a set of sealed anodized aluminum profiles with PMMA panels that are fastened together to form a work space adaptable to the desired dimensions for any laboratory environment. The size of the enclosure can be adjusted to accommodate any size equipment. The entire front of the enclosure can act as either vertical or horizontal doors which open and close very easily.

A.B. C.
Figure 5. A. Ozone Sensor B. Front Display Panel C. Filter Change

Table 1. Specifications

Dimensions, Weight & Capacity

80 cm high, 80 cm deep with 100, 150, 200 cm length
(PLUS 22,5 of the Control and catalyzer filter box)

Box and Catalytic Converting Unit

Anodized Aluminum Profiles with Transparent and Blue PMMA

Doors

PMMA plate with handles either vertical or horizontal according to the user needs

Catalytic Monolith

Dimensions

125 x 125mm

Thickness

15mm

Type

Aluminum filter using MnO2

Air Flow

1.5 m/s

High Precision Sensor

Mounted on support inside the enclosure connection to display via RJ45 connection

Accuracy

+- 0.1 parts per billion

Calibrated Range

0.000 – 0.150 ppm

Response Time

Less than 70 seconds

Operating Temperature

-5 to 50 degrees Celsius

Power Supply

12 VDC, 800 mA

Fan

1650 tr/mn – 150 W Adjustable speed, dust and particle filter

Digital Display

Realtime monitoring of ozone levels with a programmable alarm

Electrical Requirements

Power 105 W, Voltage 220V

Switch

ON/OFF located in the front of the unit

Ordering Information:

Catalog ID

Description

Price (US dollars)*

OFB100

80 cm x 80 cm x 100 cm ozone and dust free working area
(PLUS 22.5 of the Control and catalyzer filter box)

$16,000.00

OFB150

80 cm x 80 cm x 150 cm ozone and dust free working area
(PLUS 22.5 of the Control and catalyzer filter box)

$17,000.00

OFB200

80 cm x 80 cm x 200 cm ozone and dust free working area
(PLUS 22.5 of the Control and catalyzer filter box)

$18,000.00

CCF

Replacement catalyzer filter, lasts two years
12.5cm x 1.5 cm x 12.5 cm

$250.00

For more information email arrayit@arrayit.com or call 408-744-1331.

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

Warranty
ArrayIt® brand products are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All TeleChem products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with these products should be reported to TeleChem immediately. Our liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and TeleChem makes no warranty or guarantee under such circumstances.