Products - DNA Purification - Plasmid DNA Purification Kits for Rapid Column and Plate-Based Plasmid DNA Purification
Arrayit offers high quality plasmid purification kits for microarray experimentation and other life sciences applications.
Table of Contents
- Quality Control
- Product Description
- Technical Assistance
- Short Protocol
- Equipment and Reagents
- Troubleshooting Tips
- Scientific Publications
- Ordering Information
- Storage Conditions
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains a complete set of protocols including the steps and principles needed to use Arrayit’s ArrayIt® brand Plasmid DNA Mini-Prep.
Arrayit assures the performance of these products. The finest scientific research went into the development of these products. Rigorous quality control monitoring on a lot-by-lot basis guarantees that the columns, plates, buffers, and protocols conform to the highest industry standards.
Figure 1. Showing the high reliability of the kit, the error is < 5%.
- 20% higher yield than other kits
- Quicker protocol
- High quality of preps (all downstream applications)
- Processing of up to 6 ml culture
- For E. coli and other Gram-negative bacteria
Figure 2. Comparison of the ArrayIt kit to the leading competitor.
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: firstname.lastname@example.org (under the subject heading please type ArrayIt technical assistance). By telephone: (408) 744-1331, Monday—Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!
- Centrifuge culture at >13,000 rpm for 30-60 s.
- Resuspend pellet in 250 µl buffer 1.
- Mix with 250 µl buffer 2.
- Mix with 350 µl buffer 3.
- Centrifuge at >13,000 rpm for 7 min.
- Decant supernatant in mini Spin column and centrifuge at >13,000 rpm for 30 s.
- Wash column with 700 µl buffer 4 (30 s, >13,000 rpm).
- Dry membrane at >13,000 rpm for 1 min.
- Pipette 30-100 µl buffer 5 or water onto membrane, incubate for 1 min and centrifuge at >13,000 rpm for 1 min.
Time: 15 min for 2 x 2 ml culture; 45 min for 20 parallel preparations
Figure 3. Showing the high quality of plasmid DNA.
Figure 4. Plasmid Mini Prep works great for both low copy and high molecular size plasmids.
Figure 5. Useful for all E. coli cloning strains.
Figure 6. Data from low copy number plasmids from E. coli and other bacteria.
Equipment and Reagents
Arrayit products used in plasmid scientific publications.
*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.
Price (US dollars)*
Plasmid DNA Purification Kit
50 reactions in a single tube kit, reagents, buffers, columns and handbook.
Plasmid DNA Purification Kit
250 reactions in a single tube kit, reagents, buffers, columns and handbook.
10-49 kits, 10% discount
50-99 kits, 15% discount
100+ kits, 20% discount
*To order ArrayIt® Brand Products: call 408-744-1331, FAX 408-744-1711 or click on the purchase button to proceed directly to the purchase page.
Kits should be stored dry at room temperature (20-25°C). The kits perform well across a wide range of ambient temperatures and relative humidity. The kits are nuclease-free, sterile, and have a shelf life of one-year from the date of purchase.
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with our ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product including deviations from our protocols is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.
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