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Products - DNA Purification - Microarray Probe Purification Single Column and Plate-Based Kits to Purify Fluorescent Samples for Microarray Hybridization Reactions

Arrayit-FPP
Microarray Probe Purification Kit (FPP) single column version, including the Binding Buffer, Wash Buffer, Spin Columns and Wash Microfuge Tubes. Each FPP kit allows purification of 50 fluorescent labeling reactions for microarray applications. FPP kits are also available in both 96-Well and 384-Well Microplate versions for high-throughput applications.

Table of Contents

  • Introduction
  • Quality Control
  • Product Description
  • Technical Assistance
  • Short Protocol (single columns)
  • Complete Protocol (single columns)
  • Short Protocol (96 well format)
  • Complete Protocol (96 well format)
  • Short Protocol (384 well format)
  • Complete Protocol (384 well format)
  • Troubleshooting Tips
  • Kit Contents
  • Scientific Publications
  • Ordering Information
  • Storage Conditions
  • Warranty

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains a complete set of protocols including the steps and principles needed to use Arrayit’s ArrayIt® brand Fluorescent Probe Purification Kits.

Quality Control
Arrayit assures the performance of these products.  The finest scientific research went into the development of these products. Rigorous quality control monitoring on a lot-by-lot basis guarantees that the columns, microplates, buffers, and protocols conform to the highest industry standards.

Product Description
Arrayit’s ArrayIt® brand Microarray Probe Purification Kits offer advanced purification systems that use sophisticated separation technology in both single column and 384-well formats. Our Purification Kits will increase the quality of your DNA microarray data by removing unwanted salts, enzymes, primers, unincorporated dyes and nucleotides, and other contaminants that diminish the quality of DNA Hybridization reactions and elevate fluorescent background.

Users will appreciate the following features:

  • Purifies single- and double-stranded DNA and RNA probes
  • Replaces Qiagen’s QIAquick product in all protocols
  • Efficient purification of products ranging from 50-5,000 nt or bp
  • Compatible with oligo-dT and random primer protocols
  • Supports all substrate and slide surface chemistries
  • Can be used with both two- and three-dimensional surfaces
  • Superior separation chemistry provides 99+% probe purity
  • Dye removal reduces background in microarray hybridizations
  • No alcohol precipitations required
  • Glass fiber membrane improves yield and reduces contamination
  • Kits arrive ready to use, no buffer or column preparation required
  • Microcentrifuge format allows rapid purification with single columns
  • 384-well Microplate format provides high throughput purification

Compatible with the following applications:

  • Direct fluorescent labeling of total RNA and polyA+ mRNA into cDNA
  • Indirect aminoallyl labeling of RNA to make cDNA
  • Random primer labeling of genomic DNA for comparative genomic hybridization (CGH)
  • T3 and T7 RNA polymerase products
  • Many others

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance.  By electronic mail: arrayit@arrayit.com (under the subject heading please type ArrayIt technical assistance).  By telephone: (408) 744-1331, Monday-Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

probe-purification
Figure 1. Arrayit’s Microarray Probe Purification Kits FPP and FPP384 (shown here) reduce background and improve signals in microarray hybridization experiments by removing unincorporated dyes from fluorescent probe labeling reactions.

Short Protocol (single columns)

  1. Obtain a 25 Ál fluorescent labeling reaction.
  2. Add 3 volumes (75 Ál) of Fluorescent Probe Binding Buffer.
  3. Mix by vortexing vigorously.
  4. Transfer sample into a Spin Column.
  5. Place the Spin Column into a Wash Microfuge Tube.
  6. Centrifuge 10 sec in a microfuge at 1,000 rpm.
  7. Discard flow-through from Wash Microfuge Tube.
  8. Add 125 Ál Wash Buffer to Spin Column.
  9. Centrifuge 10 sec in a microfuge at 1,000 rpm.
  10. Discard wash eluent from Wash Microfuge Tube.
  11. Wash two more times (repeating steps 8-10 twice).
  12. Centrifuge Spin Column 60 sec in a microfuge to dry completely.
  13. Transfer Spin Column to a sterile Elution Microfuge Tube.
  14. Add 50 Ál sterile dH20 to the Spin Column.
  15. Incubate Spin Column for 5 min at room temperature.
  16. Centrifuge Spin Column for 60 sec at 1,000 rpm in a microfuge to elute sample.

Complete Protocol (single columns)

  1. Obtain a 25 Ál fluorescent labeling reaction.  Set up and label mixtures of DNA or RNA using any protocol of choice.  Labels can be either fluorescent or non-fluorescent.  After the labeling reaction, proceed immediately to step 2.
  2. Add 3 volumes (75 Ál) of Binding Buffer. To the 25 Ál labeling reaction, add 3 volumes of Binding Buffer. The components in the Binding Buffer render nucleic acids insoluble, leaving the unwanted components (salts, enzymes, primers, fluors, etc.) in soluble form.
  3. Mix by vortexing vigorously. This step is critical. Make certain to mix the labeling reaction and the Binding Buffer thoroughly.  Failure to mix completely will reduce yield and produce weaker microarray signals.
  4. Transfer sample into a Spin Column.  Carefully pipette the mixture into the top of a white Spin Column, making sure to aliquot the sample directly onto the filter membrane.  Insoluble nucleic acids will bind tightly to the filter membrane, allowing contaminants to flow through the Spin Column.
  5. Place the Spin Column into a Wash Microfuge Tube.  Insert the bottom end of the Spin Column into a Wash Microfuge Tube.  The Spin Column should fit snuggly, allowing centrifugation.
  6. Centrifuge 10 sec in a microfuge at 1,000 rpm. This step traps the insoluble nucleic acids onto the filter membrane of the Spin Column, allowing contaminants to pass into the Wash Microfuge Tube.
  7. Discard flow-through from Wash Microfuge Tube. After the 10 sec spin step, empty the contents of the Wash Microfuge Tube by inverting the tube. Excess liquid can be removed by blotting the top of the Wash Microfuge Tube onto an absorbant wipe (e.g. bench coat, cleanroom wipes, etc.).
  8. Add 125 Ál Wash Buffer to Spin Column. Pipette 125 Ál of Wash Buffer into the Spin Column.  Wash Buffer solubilizes residual contaminants on the Spin Column membrane.
  9. Centrifuge 10 sec in a microfuge at 1,000 rpm. This step moves the Wash Buffer through the Spin Column membrane into the Wash Microfuge Tube.
  10. Discard wash eluent from Wash Microfuge Tube.  After the 10 second spin step, empty the contents of the Wash Microfuge Tube by inverting the tube. Excess liquid can be removed by blotting the top of the Wash Microfuge Tube onto an absorbant wipe such as a Microarray Cleanroom Wipe.
  11. Wash two more times (repeating steps 8-10 twice).  By repeating steps 8-10 two more times, all remaining contaminants are removed from the Spin Column membrane, leaving highly purified labeled nucleic acids.
  12. Centrifuge Spin Column 60 sec in a microfuge to dry completely.  This step removes residual Wash Buffer from the membrane, aiding in the elution step.
  13. Transfer Spin Column to a sterile Elution Microfuge Tube.  Place the Spin Column containing the bound sample into an Elution Microfuge tube and press it firmly into place. The Spin Column should fit snuggly inside the Elution Microfuge Tube. 
  14. Add 50 Ál sterile dH20 to the Spin Column.  Pipette a 50 Ál aliquot of sterile dH20 directly onto the Spin Column membrane, to re-solubilize the bound nucleic acid.
  15. Incubate Spin Column for 5 min at room temperature.  Allow the nucleic acid in the Spin Column to re-solubilize without agitation for 5 minutes.  Do not attempt to centrifuge the sample prior to the 5-minute incubation, as this will reduce yield.
  16. Centrifuge Spin Column for 60 sec at 1,000 rpm in a microfuge to elute sample. Place the Elution Microfuge Tube containing the Spin Column and 50 Ál dH20 into a microfuge (see Fig. 2), and spin at 1,000 rpm for 1 minute.  Make certain that the entire 50 Ál volume has passed through the membrane and into the Elution Microfuge Tube.  The eluent contains the fluorescent nucleic acid molecules bound previously to the Spin Column membrane.
  17. Dry fluorescent sample in a SpeedVac for 30 min at 42░C. Remove the dH20 from the purified fluorescent sample by drying it for 30 min at elevated temperature (37-65░C) in a centrifuge equipped with a vacuum. The purified sample will appear as a small pellet at the bottom of the Elution Microfuge Tube.
  18. Re-suspend fluorescent sample in hybridization buffer of choice. Add the appropriate volume of dH20 and hybridization buffer to re-suspend the fluorescent sample.  Arrayit’s Hybridization Buffers work extremely well for all DNA microarray applications.
  19. Store at –20░C until ready to use. Fluorescent and other types of labeled samples should be stored at –20░C or –80░C until use.  Many fluorescent molecules are sensitive to light and should be stored in the dark.

fluorescent-labeling-kit
Figure 2. Shown are 12 FPP Spin Columns in a microfuge. Each Spin Column was loaded with a 100 Ál fluorescent sample obtained by mixing a 25 Ál probe labeling reaction with 75 Ál of Binding Buffer.  The Wash Microfuge Tubes collect the eluent from the Spin Columns during the centrifugation steps.

fluorescent-purification-kit
Figure 3. Shown are labeled samples being transferred into a 384-Well Filter Microplate. The FPP384 kit allows users to purify fluorescent probes in a 384-well format, and the FPP384 kit can be used manually (shown here) or with an automated liquid handling device.

Short Protocol (96-well format, 50 Ál reactions)

  1. Obtain 50 Ál labeling reactions.
  2. Add 150 Ál or 3 volumes of Binding Buffer.
  3. Mix thoroughly by pipetting or vortexing.
  4. Transfer samples to the 96-Well Filter Microplate.
  5. Place the Filter Microplate onto a Wash Microplate.
  6. Centrifuge the microplates for 1 min at 500 x g to elute contaminants and Binding Buffer.
  7. Discard the eluent from the Wash Microplate.
  8. Wash 3 times with 250 Ál of Wash Buffer using the Wash Microplate to collect the eluent.
  9. Remove residual Wash Buffer by centrifugation for 5 min at 500 x g.
  10. Incubate the Filter Microplate for 5 min additional minutes to allow drying.
  11. Place the Filter Microplate onto an Elution Microplate.
  12. Add 100 Ál of dH20 to each well.
  13. Incubate for 5 min at room temperature to allow sample re-hydration.
  14. Centrifuge the microplates for 1 min at 500 x g to elute the purified samples into the Elution Microplate (lower microplate).
  15. Discard the Filter Microplate (upper microplate).
  16. Dry the samples by vacuum centrifugation for 30 min at 42░C.
  17. Re-suspend the fluorescent samples in hybridization buffer.
  18. Store at –20░C until ready to use.

Complete Protocol (96-well format, 50 Ál reactions)

  1. Obtain 50 Ál labeling reactions. Samples can be DNA, total RNA, mRNA, or any other type of nucleic acid used for microarray probes. Reaction volumes can be scaled up or down as needed. One protocol recommended by the OCI (see technical note) labels mRNA with reverse transcriptase in a 40 Ál volume.  The reaction is terminated by adding 4 Ál of 50 mM EDTA and the mRNA is degraded by adding 2 Ál 10 N NaOH, followed by heating for 20 min at 65░C. After the 20 min mRNA degradation step, the sample is neutralized with 4 Ál of 5 M acetic acid.
  2. Add 150 Ál or 3 volumes of Binding Buffer. To the 50 Ál labeling reactions, add 3 volumes of Binding Buffer. For a 50 Ál labeling reaction add 150 Ál of Binding Buffer. For reactions of lesser volume, add 3 volumes of Binding Buffer. The components in the Binding Buffer render nucleic acids insoluble, leaving the contaminants (salts, enzymes, primers, fluors, etc.) in soluble form so that the pass through the filter.
  3. Mix thoroughly by pipetting or vortexing.  This step is critical.  Make certain to mix the labeling reactions and the Binding Buffer thoroughly.  Failure to mix completely will reduce yield and produce weaker microarray signals. Care should be taken not to splash samples from well to well, if labeling and mixing is performed in a Microplate format.
  4. Transfer samples to the 96-Well Filter Microplate.  Carefully pipette the mixtures into separate wells of the Filter Microplate, making sure to aliquot the sample directly onto the filter membrane. Insoluble nucleic acids will bind tightly to the filter membrane, allowing contaminants to flow through the Filter Microplate.
  5. Place the Filter Microplate onto a Wash Microplate.  Insert the drip directors on the bottom of the Filter Microplate into the corresponding wells of the Wash Microplate (see Fig. 3), making certain to use the correct microplate orientation.  The Filter and Wash Microplates should form a tightly fitting “sandwich” that is readily centrifuged. Do not orient the Filter and Wash Microplates incorrectly or attempt to centrifuge incorrectly registered microplates.
  6. Centrifuge the microplates for 1 min at 500 x g to elute contaminants and Binding Buffer.  This step traps the insoluble nucleic acids onto the membrane of the Filter Microplate, allowing contaminants to pass into the Wash Microplate.  This step can also be performed using a vacuum apparatus and a gentle vacuum for filtration. For the vacuum-based approach, a Wash Microplate is not required during filtration, as the eluent is collected in the reservoir of the vacuum apparatus.
  7. Discard the eluent from the Wash Microplate. Invert the Wash Microplate and discard the eluent.  Residual eluent can be removed from the Wash Microplate by blotting it onto an absorbant wipe such as Microarray Cleanroom Wipe.
  8. Wash 3 times with 250 Ál of Wash Buffer using the Wash Microplate to collect the eluent.  This can be performed using a multi-channel pipette or automated liquid handling device. Centrifugation for 1 min at 500 x g is used to pass the Wash Buffer through the Filter Microplate and into the Wash Microplate. The eluent is discarded after each centrifugation step.  Residual eluent can be removed from the Wash Microplate by blotting it onto an absorbant wipe such as Microarray Cleanroom Wipe.
  9. Remove residual Wash Buffer by centrifugation for 5 min at 500 x g.  This step removes any remaining Wash Buffer trapped on the membrane of the Filter Microplate.  This step can also be performed using a strong vacuum for 5 minutes.
  10. Incubate the Filter Microplate for 5 min additional minutes to allow drying. This room temperature (20-30░C) incubation produces a slight yield in purified sample, by allowing additional drying of the membrane in the Filter Microplate. Residual Wash Buffer on membrane can reduce yield by slowing the re-suspension of the probe molecules bound to the membrane.
  11. Place the Filter Microplate onto an Elution Microplate.  Insert the drip directors on the bottom of the Filter Microplate into the corresponding wells of the Elution Microplate (see Fig. 3), making certain to use the correct microplate orientation. The Filter and Elution Microplates should form a tightly fitting “sandwich” that is readily centrifuged. Do not orient the Filter and Wash Microplates incorrectly or attempt to centrifuge incorrectly registered microplates.
  12. Add 100 Ál of dH20 to each well.  The addition of 100 Ál of dH20 re-solubilizes probe molecules trapped on the Filter Microplate membrane. Make certain to pipette the dH20 directly onto the membrane surface.  Do not agitate the Filter Microplate during this step.
  13. Incubate for 5 min at room temperature to allow sample re-hydration. The 5 minute incubation allows time for the precipitated molecules to re-suspend. Do not attempt to centrifuge the microplates before the 5 min incubation, as this will reduce yield.
  14. Centrifuge the microplates for 1 min at 500 x g to elute the purified samples into the Elution Microplate (lower microplate). The 100 Ál sample will pass from the upper microplate into the lower microplate.  The final sample volume will be 80-100 Ál.
  15. Discard the Filter Microplate (upper microplate).  Following the elution step, discard the Filter Microplate. Arrayit does not recommend the re-use of Filter Microplates, as this can cause sample contamination.
  16. Dry the samples by vacuum centrifugation for 30 min at 42░C. The 80-100 Ál samples should be taken to dryness, forming a small pellet on the bottom of each well.  A centrifugation temperature of 37-65░C can be used for the drying step.
  17. Re-suspend the fluorescent samples in hybridization buffer.  Samples should be re-suspended in the appropriate volume of dH20 and hybridization buffer, and Arrayit makes a complete line of Hybridization Buffers for all DNA microarray applications.
  18. Store at –20░C until ready to use. Purified probes should be stored at –20░C or –80░C in the dark, until use.

Short Protocol (384 well format, 25 Ál reactions)

  1. Obtain 25 Ál labeling reactions.
  2. Add 75 Ál or 3 volumes of Binding Buffer.
  3. Mix thoroughly by pipetting or vortexing.
  4. Transfer samples to the 384-Well Filter Microplate.
  5. Place the Filter Microplate onto a Wash Microplate.
  6. Centrifuge the microplates for 1 min at 500 x g to elute contaminants and Binding Buffer.
  7. Discard the eluent from the Wash Microplate.
  8. Wash 3 times with 75 Ál of Wash Buffer using the Wash Microplate to collect the eluent.
  9. Remove residual Wash Buffer by centrifugation for 5 min at 500 x g.
  10. Incubate the Filter Microplate for 5 min additional minutes to allow drying.
  11. Place the Filter Microplate onto an Elution Microplate.
  12. Add 25 Ál of dH20 to each well.
  13. Incubate for 5 min at room temperature to allow sample re-hydration.
  14. Centrifuge the microplates for 1 min at 500 x g to elute the purified samples into the Elution Microplate (lower microplate).
  15. Discard the Filter Microplate (upper microplate).
  16. Dry the samples by vacuum centrifugation for 30 min at 42░C.
  17. Re-suspend the fluorescent samples in hybridization buffer.
  18. Store at –20░C until ready to use.

Complete Protocol (384 well format, 25 Ál reactions)

  1. Obtain 25 Ál labeling reactions. Samples can be DNA, total RNA, mRNA, or any other type of nucleic acid used for microarray probes. Reaction volumes can be scaled up or down as needed. One protocol recommended by the OCI (see technical note) labels mRNA with reverse transcriptase in a 40 Ál volume.  The reaction is terminated by adding 4 Ál of 50 mM EDTA and the mRNA is degraded by adding 2 Ál 10 N NaOH, followed by heating for 20 min at 65░C. After the 20 min mRNA degradation step, the sample is neutralized with 4 Ál of 5 M acetic acid.
  2. Add 75 Ál or 3 volumes of Binding Buffer.  To the 25 Ál labeling reactions, add 3 volumes of Binding Buffer. The components in the Binding Buffer render nucleic acids insoluble, leaving the unwanted components (salts, enzymes, primers, fluors, etc.) in soluble form.
  3. Mix thoroughly by pipetting or vortexing.  This step is critical.  Make certain to mix the labeling reactions and the Binding Buffer thoroughly.  Failure to mix completely will reduce yield and produce weaker microarray signals. Care should be taken not to splash samples from well to well, if labeling and mixing is performed in a Microplate format.
  4. Transfer samples to the 384-Well Filter Microplate.  Carefully pipette the mixtures into separate wells of the Filter Microplate, making sure to aliquot the sample directly onto the filter membrane. Insoluble nucleic acids will bind tightly to the filter membrane, allowing contaminants to flow through the Filter Microplate.
  5. Place the Filter Microplate onto a Wash Microplate.  Insert the drip directors on the bottom of the Filter Microplate into the corresponding wells of the Wash Microplate (see Fig. 3), making certain to use the correct microplate orientation.  The Filter and Wash Microplates should form a tightly fitting “sandwich” that is readily centrifuged. Do not orient the Filter and Wash Microplates incorrectly or attempt to centrifuge incorrectly registered microplates.
  6. Centrifuge the microplates for 1 min at 500 x g to elute contaminants and Binding Buffer.  This step traps the insoluble nucleic acids onto the membrane of the Filter Microplate, allowing contaminants to pass into the Wash Microplate.  This step can also be performed using a vacuum apparatus and a gentle vacuum for filtration. For the vacuum-based approach, a Wash Microplate is not required during filtration, as the eluent is collected in the reservoir of the vacuum apparatus.
  7. Discard the eluent from the Wash Microplate. Invert the Wash Microplate and discard the eluent.  Residual eluent can be removed from the Wash Microplate by blotting it onto an absorbant wipe such as Microarray Cleanroom Wipe.
  8. Wash 3 times with 75 Ál of Wash Buffer using the Wash Microplate to collect the eluent.  This can be performed using a multi-channel pipette or automated liquid handling device. Centrifugation for 1 min at 500 x g is used to pass the Wash Buffer through the Filter Microplate and into the Wash Microplate.  The eluent is discarded after each centrifugation step.  Residual eluent can be removed from the Wash Microplate by blotting it onto an absorbant wipe such as Microarray Cleanroom Wipe.
  9. Remove residual Wash Buffer by centrifugation for 5 min at 500 x g.  This step removes any remaining Wash Buffer trapped on the membrane of the Filter Microplate.  This step can also be performed using a strong vacuum for 5 minutes.
  10. Incubate the Filter Microplate for 5 min additional minutes to allow drying. This room temperature (20-30░C) incubation produces a slight yield in purified sample, by allowing additional drying of the membrane in the Filter Microplate. Residual Wash Buffer on membrane can reduce yield by slowing the re-suspension of the probe molecules bound to the membrane.
  11. Place the Filter Microplate onto an Elution Microplate.  Insert the drip directors on the bottom of the Filter Microplate into the corresponding wells of the Elution Microplate (see Fig. 3), making certain to use the correct microplate orientation. The Filter and Elution Microplates should form a tightly fitting “sandwich” that is readily centrifuged. Do not orient the Filter and Wash Microplates incorrectly or attempt to centrifuge incorrectly registered microplates.
  12. Add 25 Ál of dH20 to each well.  The addition of 25 Ál of dH20 re-solubilizes probe molecules trapped on the Filter Microplate membrane. Make certain to pipette the dH20 directly onto the membrane surface.  Do not agitate the Filter Microplate during this step.
  13. Incubate for 5 min at room temperature to allow sample re-hydration. The 5 minute incubation allows time for the precipitated molecules to re-suspend. Do not attempt to centrifuge the microplates before the 5 min incubation, as this will reduce yield.
  14. Centrifuge the microplates for 1 min at 500 x g to elute the purified samples into the Elution Microplate (lower microplate). The 25 Ál sample will pass from the upper microplate into the lower microplate.  The final sample volume will be 20-25 Ál.
  15. Discard the Filter Microplate (upper microplate).  Following the elution step, discard the Filter Microplate. Arrayit does not recommend the re-use of Filter Microplates, as this can cause sample contamination.
  16. Dry the samples by vacuum centrifugation for 30 min at 42░C. The 20-25 Ál samples should be taken to dryness, forming a small pellet on the bottom of each well.  A centrifugation temperature of 37-65░C can be used for the drying step.
  17. Re-suspend the fluorescent samples in hybridization buffer.  Samples should be re-suspended in the appropriate volume of dH20 and hybridization buffer, and Arrayit makes a complete line of Hybridization Buffers for all DNA microarray applications.
  18. Store at –20░C until ready to use. Purified probes should be stored at –20░C or –80░C in the dark, until use.

Technical note. Helpful suggestions to Arrayit’s standard protocols were provided courtesy of the Ontario Cancer Institute and the University Health Network Microarray Centre.  The FPP and FPP384 kits work well with Cy3- and Cy5-labeled samples, and the OCI recommends the use of reciprocal labeling experiments to minimize signal variation attributable to minor differences in incorporation, quantum yield, and dye stability.  The OCI prefers the FPP384 kits to the single spin column format (FPP) for reasons of ease-of-use, throughput, and affordability. The OCI emphasizes that the 384-well microplates can be used for small sets of samples over days, weeks, or even months, by using a subset of micomicroplate wells for each successive batch of fluorescent samples.

fluorescent-probe-purification
Figure 4. Microarray data with FPP384 purified probes. Flourescent probes were labeled with Cy3 and Cy5, purified with Arrayit’s 384-well Fluorescent Probe Purification Kit (FPP384), and hybridized to a human cDNA microarray. The microarray was scanned for Cy3 emission (left panel) and Cy5 emission (right panel), with data coded to a rainbow palette. The intense fluorescent signals and low background are readily observed with the FPP384-purified probes.  Sample data provided by the Ontario Cancer Institute.

Equipment and Reagents
Super Microarray Substrates
Mirror Substrates
Hybridization Solutions
Hybridization Cassettes
Microarray Instruments
Cleanroom Wipes

Troubleshooting Tips
Low fluorescent signals on microarray

  • Sample did not label efficiently
  • Inefficient elution of fluorescent products from spin column or Microplate

High fluorescent background on microarray

  • Hybridization reaction dehydrated during hybridization reaction. Use Arrayit hybridization cassettes and hybridization solutions for optimal results
  • Fluorescent probe mixture not washed properly. Follow the FPP and FPP384 protocol exactly as written above

Kit Contents
Single column (FPP)

  • Spin Columns (50)
  • Wash Microfuge Tubes (50)
  • Elution Microfuge Tubes (50)
  • Binding Buffer (30 ml)
  • Wash Buffer (30 ml)
  • Handbook (1)

96-well microplate, 50 Ál reactions (FPP96)

  • 96-Well Filter Microplate (1)
  • Wash Microplate (1)
  • Elution Microplate (1)
  • Binding Buffer (30 ml)
  • Wash Buffer (125 ml)
  • Handbook (1)

96-well microplate, 100 Ál reactions (FPP96100)

  • 96-Well Filter Microplate (1)
  • Wash Microplate (1)
  • Elution Microplate (1)
  • Binding Buffer (60 ml)
  • Wash Buffer (250 ml)
  • Handbook (1)

384-well microplate, 25 Ál reactions (FPP384)

  • 384-Well Filter Microplate (1)
  • Wash Microplate (1)
  • Elution Microplate (1)
  • Binding Buffer (50 ml)
  • Wash Buffer (150 ml)
  • Handbook (1)

Scientific Publications
Arrayit products featured in probe scientific publications.

Ordering Information

Product

Description

Catalog ID

Price (US dollars)*

Fluorescent Probe Purification Kit, Single Column Format

Fluorescent Probe Purification Kit (FPP) designed to purify fluorescent probe molecules from labeling reactions. Kit contains 50 single column glass fiber purification columns, wash and elution microplates, binding buffer, wash buffer and protocols, for 50 x 50 Ál labeling reactions.

FPP

$144

Fluorescent Probe Purification Kit, 96-Well 50 Ál Format

Fluorescent Probe Purification Kit (FPP) designed to purify fluorescent probe molecules from labeling reactions. Kit contains a 96-well glass fiber purification microplate, wash and elution microplates, binding buffer, wash buffer and protocols, for 96 x 50 Ál labeling reactions.

FPP96

$185

Fluorescent Probe Purification Kit, 96-Well 100 Ál Format

Fluorescent Probe Purification Kit (FPP) designed to purify fluorescent probe molecules from labeling reactions. Kit contains a 96-well deep well glass fiber purification microplate, wash and elution microplates, binding buffer, wash buffer and protocols, for 96 x 100 Ál labeling reactions.

FPP96100

$202

Fluorescent Probe Purification Kit, 384-Well 25 Ál Format

Fluorescent Probe Purification Kit (FPP) designed to purify fluorescent probe molecules from labeling reactions. Kit contains a 384-well glass fiber purification microplate, wash and elution microplates, binding buffer, wash buffer and protocols, for 384 x 25 Ál labeling reactions.

FPP384

$185

FPP Discounts
10-19 kits, 10% discount
20-50 kits, 15% discount off list price
50+ kits, 20% discount

FPP96, FPP96100 and FPP384 Discounts
10-49 kits, 20% discount
50-99 kits, 25% discount
100+ kits, 30% discount

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

*To order ArrayIt® Brand Products: call 408-744-1331, fax 408-744-1711 or click on the purchase button to proceed directly to the purchase page.

Storage Conditions
Arrayit’s ArrayIt® brand Fluorescent Probe Purification Kits should be stored dry at room temperature (20-25░C).  The kits perform well across a wide range of ambient temperatures and relative humidity.  The kits are nuclease-free, sterile, and have a shelf life of one-year from the date of purchase.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with our ArrayIt® brand product should be reported to Arrayit immediately. Arrayit’s liability is limited to the replacement of the product, or a full refund.  Any misuse of this product including deviations from our protocols is the full responsibility of the user, and Arrayit makes no guarantees under these circumstances.

 

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